Detailed Information

Research Field/Keywords for Research Field

• Biological Inorganic Chemistry, Biological Physics, Spectroscopy, Structural Biology, Enzymology

Education

• 2002/03, Osaka University, Graduated
• 2004/03, Osaka University, Graduate School of Engineering Science, Division of Bioengineering, Master Course, Completed
• 2008/03, Osaka University, Graduate School of Engineering Science, Division of Bioengineering, Doctor Course, Completed

Employment Experience

• 2008/08 - 2010/08 Research Fellow Riken, Harima Institute, SPring-8 Center
• 2010/09 - 2016/11 Research Fellow Northwestern University, Department of Chemistry
• 2016/12 - 2022/03 Assistant Professor, Department of Applied Biochemistry and Food Science, Saga University
• 2022/04 -  *  Associate Professor, Department of Applied Biochemistry and Food Science, Saga University

Field of Specialization

• biophysics, Bio-related Chemistry, structural biochemistry, Physical Chemistry

Membership in Academic Societies

• International EPR (ESR) Society

Awards

• 電子スピンサイエンス学会奨励賞 （2016/11）
• The Travel Grant of BMB2015 （2015/12）
• AAAS/Science Program for Excellence in Science （2016/06）

Themes for Ongoing Research

• Development of High-Field and Multi-Frequency EPR Spectroscopy and Study on Metalloprotein with Integer Spin System

Research Themes in the Past

• Development of High-Field and Multi-Frequency EPR Spectroscopy and Study on Metalloprotein with Integer Spin System

Research Topics and Results

• EPR Spectroscopy and X-ray Crystallography Revealed Rearrangements of Di-Mn Active Site by Substrate Binding in Inorganic Pyrophosphatase 2023/11
• Quick and Spontaneous Transformation between [3Fe-4S] and [4Fe-4S] Iron-Sulfur Clusters in the tRNA-Thiolation Enzyme TtuA 2023/01
• Intermolecular Electron Transfer from Biopterin to FeII-O2 Complex in Nitric Oxide Synthases Occurs at very Different Rates Between Bacterial and Mammalian Enzymes: Direct Observation of a Catalytically Active Intermediate 2023/01
• Trapping of Mono-Nitrosyl Non-Heme Intermediate of Nitric Oxide Reductase by Cryo-photolysis of Caged Nitric Oxide 2023/00
• Heme Protein Identified from Scaly-Foot Gastropod can Synthesize Pyrite (FeS2) Nanoparticles 2023/00
• スピンラベル-ESR法を利用した酵素の構造柔軟性の実測 2022/12
• ヘムトランスポーターの輸送機構解明へ向けた新たな反応中間体捕捉法の開発 2022/12
• 急速凍結-電子常磁性共鳴（EPR）法を用いた金属酵素活性中心における構造変化の温度依存性 2022/12
• ヘム駆動型フェロミクスプローブの反応機構解明 2022/12
• EPR Spectroscopy Reveals the Differences of Active Site Structure for Di-Mn Enzymes from Mesophillic and Psychrophillic Bacteria 2022/09
• X-ray Crystallography and Spin-Labeling ESR Reveal Cold Adaptation and High Thermal Stability Mechanism of Cold-Adapted Glucokinase 2022/09
• EPR Spectroscopy Reveals Active Site Rearrangements of Di-Mn Center in Inorganic Pyrophosphatase 2022/09
• 南極海由来金属酵素の酵素反応・低温適応機構 2022/09
• 先端X線回折法によるグルコキナーゼの動的構造-機能相関の解明 2022/09
• 反応中間体捕捉から解き明かすミモシン合成酵素の2機能性獲得機構 2022/09
• 先端ESR法と生命科学研究への潮流 2022/09
• X線結晶構造解析による可溶性Family II無機ピロフォスファターゼの触媒活性及び構造ダイナミクス機構の解明 2022/09
• 電子スピン共鳴法で”観る”金属酵素活性部位の温度による微細構造変化 2022/07
• スピンラベル-ESR法を利用した酵素の構造柔軟性の実測 2022/07
• ヘムタンパク質の科学、第5章 測定解析技術・第3節 "磁気共鳴EPRでヘムタンパク質の何をみるか？―活性中心の電子状態とミクロ分子環境―" 2022/05
• 生命金属動態の解明に向けた光照射EPR技術による新しい酵素反応反応機構法の開発 2022/05
• X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy Reveal Active Site Rearrangement of Cold Adaptation Enzyme 2021/12
• Crystal Structure of Soluble Family II Inorganic Pyrophosphatase Revealed the Mechanism of Catalysis and Structure Dynamics 2021/11
• Structural Insights into Catalytic Reaction Mechanism for bifunctional enzyme, Mimosine Synthase, from Leucaena leucocephala 2021/11
• X-ray crystallography and spin-labeling ESR reveal cold adaptation and high thermal stability mechanisms of cold-adapted glucokinase 2021/11
• The molecular mechanism of phytosphingosine binding to FFAR4/GPR120 differs from that of other fatty acids 2021/11
• 急速凍結XAFS法による低温適応無機ピロフォスファターゼの温度特性機構の解明 2021/09
• Analysis on Cold Adaptation Mechanism of Metalloenzyme by X-ray Crystallography and EPR Spectroscopy Combined with Rapid Freeze-Quench 2021/08
• X-ray Crystallography and Site-Directed Spin-Labeling ESR Reveal Cold Adaptation and High Thermal Stability Mechanisms of Cold-Adapted Glucokinase 2021/08
• Biochemical Properties of CumA Multicopper Oxidase from Plant Pathogen, Pseudomonas syringae 2021/07
• 動的・静的構造解析による南極産好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明 2021/06
• 急速凍結XAFS分光法による低温適応無機ピロフォスファターゼの金属活性中心の局所構造解析 2021/06
• X線結晶構造解析法による低温適応無機ピロフォスファターゼの触媒活性機構の解明 2021/06
• 急速凍結EPR法およびX線結晶構造解析法による好冷細菌由来無機ピロフォスファターゼの低温適応機構の解明 2021/06
• Advanced High Field and THz ESR Spectroscopic Studies on Heme Proteins with Integer Spin System 2021/03
• 生命金属ダイナミクス～生体内における金属の挙動と制御～ 2021/01
• 高速混合凍結EPR法による低温適応酵素の低温適応機構解析 2020/11
• 整数スピン系ヘムタンパク質研究に応用可能な先端テラヘルツESR装置開発 2020/11
• 好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明 2020/11
• X線結晶構造解析および高速混合凍結・電子常磁性共鳴法による南極由来低温適応酵素の静的・動的構造解析 2020/10
• 南極産好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明 2020/10
• 南極産好冷細菌由来ピロフォスファターゼのX線結晶構造解析による低温適応機構の解明 2020/10
• X-ray Crystallography and EPR Spectroscopy Reveal Active Site Rearrangement of Cold-Adapted Inorganic Pyrophosphatase 2020/09
• Cold Adaptation and High Thermal Stability Mechanism of Glucokinase from psychrophillic bacteria are revealed by spin-labeling ESR 2020/09
• Timing of NO Binding and Protonation in the Catalytic Reaction of Bacterial Nitric Oxide Reductase as Established by Time-Resolved Spectroscopy 2020/07
• The [4Fe-4S] Cluster of Sulfurtransferase TtuA Desulfurizes TtuB during tRNA Modification in Thermus Thermophilus 2020/04
• X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy Reveal Active Site Rearrangement of Cold-Adapted Inorganic Pyrophosphatase 2020/03
• 好冷細菌由来無機ピロフォスファターゼの低温適応機構・熱安定性機構 2019/12
• 好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明 2019/12
• An Ancient Type of MnmA Protein is an Iron-Sulfur Cluster-Dependent Sulfurtransferase for tRNA Anticodons 2019/12
• 好冷細菌由来無機ピロフォスファターゼの低温適応機構 2019/11
• 鉄硫黄クラスターが関与するtRNAチオ化修飾酵素の詳細な反応機構解明を目指した研究 2019/11
• ラジカルSAM酵素におけるラジカル反応機構～自然界はどのようにしてラジカルを安全に利用しているか～ 2019/09
• Cold Adaption Inorganic Pyrophosphatase from Shewanella sp. AS-11 was Investigated the Reaction Mechanism by X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy 2019/08
• 先端テラヘルツESR装置開発によるヘムタンパク質研究の新展開 2019/07
• Electron Paramagnetic Resonance Reveals Cold Adaption Mechanism of the Psychrophilic Metalloenzyme 2019/06
• Development of Advanced THz ESR Spectroscopy for Heme Proteins with Integer Spin System 2019/06
• EPR Spectroscopy Reveals the Active Site Rearrangement of Di-Mn2+ Center by Substrate Binding in Inorganic Pyrophosphatase from Shewanella sp. AS-11 2019/06
• Electron Paramagnetic Resonance Spectroscopy Investigates Di-Manganese(II) Active Site of Inorganic Pyrophosphate from Shewanella sp. AS-11 2018/10
• Structural Analysis on di-Mn ion site for Shewanella inorganic pyrophosphatase by EPR 2018/09
• EPR Studies Reveal Mn(II)-Mn(II) Distance in the Active Site of Inorganic Pyrophosphatase from Shewanella sp. AS-11 2018/09
• 低温適応酵素グルコキナーゼの低温機構解明 2018/08
• 電子常磁性共鳴法による低温適応酵素無機ピロフォスファターゼの複核Mnイオンサイトの構造解析 2018/07
• ENDOR 法によるタンパク質‐基質複合体精密構造解析　～酵素によるトンネリング機構解明へ～ 2017/11
• 低温適応酵素グルコキナーゼの低温機構解明に向けて 2017/11
• ラジカルSAM酵素はなぜ自然界に幅広く受け入れられてきたか～先端磁気共鳴法による酵素反応解析～ 2017/09
• Substrate Positioning of Soybean Lipoxygenase For H-Atom Abstraction by ENDOR 'Crystrallography' 2017/09
• Trapping of the Reaction Intermediates and Analysis of Enzymatic Catalytic reaction on Radical SAM Enzymes 2017/08
• Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme 2017/08
• 電子スピン共鳴法による低温適応無機ピロフォスファターゼ活性部位Mn2+イオンの電子状態解析 2017/08
• 生物学的プロトントンネリング機構解明へ～新規ENDOR解析法によるタンパク質構造精密解析 2017/07
• ENDOR Characterization of an Iron-Alkene Complex Provides Insight into a Corresponding Organometallic Intermediate of Nitrogenase 2017/06
• Substrate Positioning for H-Atom Abstraction in Soybean Lipoxygenase by ENDOR Crystallography 2017/06
• New ENDOR Analysis Reveals the Structural Basis for C-H Activation by Biological Tunneling in Soybean Lipoxygenase 2017/06
• Substrate-Dependent Cleavage Site Selection by Unconventional Radical S-Adenosylmethionine Enzymes in Diphthamide Biosynthesis 2017/04
• 先端磁気共鳴法による金属タンパク質研究 2017/04
• ¹³C ENDOR spectroscopy of lipoxygenase-substrate complexes reveals the structural basis for C-H activation by tunneling 2017/02
• 先端磁気共鳴法によるラジカルSAM酵素の反応機構 2017/01
• ENDOR reveals how radical SAM enzymes tame highly reactive radical 2016/11
• 先端磁気共鳴法による金属タンパク質研究 2016/11
• Negative cooperativity in the nitrogenase Fe protein electron delivery cycle 2016/10
• Organometallic Complex Formed by an Unconventional Radical S-Adenosylmethionine Enzyme 2016/08
• Radical SAM Catalysis via an Organometallic Intermediate with an Fe-[5’C-Deoxyadenosyl] Bond 2016/06
• ラジカルSAM酵素における有機金属反応中間体の観測 2016/06
• Metalloproteins: Radical SAM catalysis via an organometallic intermediate with an Fe-[5′-C]-deoxyadenosyl bond 2016/05
• Investigating the position of the hairpin loop in New Delhi metallo-β-lactamase, NDM-1, during catalysis and inhibitor binding 2016/03
• Why Nature Uses Radical SAM Enzyme Superfamily so Widely 2015/12
• Why Nature Uses Radical SAM Enzymes so Widely: Electron Nuclear Double Resonance Studies of Lysine 2,3-Aminomutase Show the 5′-dAdo• "free Radical" Is Never Free 2015/06
• Why Nature Uses Radical SAM Enzymes so Widely ~ENDOR Studies on Lysine 2,3-Aminomutase Show the 5’-dAdo• ‘Free Radical’ is Never Free~ 2015/06
• ENDOR法によるラジカルSAM酵素の反応機構解明-なぜ生物はラジカルSAM酵素を広く利用するのか？- 2015/06
• New Approach to ENDOR Analysis for Manganese(II)-Substituted Soybean Lipoxygenase with A Non-Coordinated Substrate 2014/11
• The Structural Analysis for Reaction Intermediate of Lysine 2,3-Aminomutase by ENDOR Spectroscopy 2012/08
• Characterization of the Fe-H bond in a three-coordinate terminal hydride complex of iron(I) 2012/04
• Low-lying electronic states of the ferrous high-spin (S = 2) heme in deoxy-Mb and deoxy-Hb studied by highly-sensitive multi-frequency EPR 2011/12
• Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2,3-dioxygenase during catalytic turnover 2011/12
• Resonance Raman identification of a FeIV=O type reaction intermediate during indoleamine 2, 3-dioxygenase reaction 2010/12
• Identification of the Fe-O 2 and the Fe=O heme species for indoleamine 2,3-dioxygenase during catalytic turnover 2010/02
• Multi-frequency and high-field EPR study of manganese(III) protoporphyrin IX reconstituted myoglobin with an S = 2 integer electron spin 2008/04
• 多周波数・強磁場EPR(MFEPR)法を用いた整数スピン系金属タンパク質研究の新たな展開 ～　Mn3+ポルフィリン置換ミオグロビン(S = 2)　～ 2007/04
• Development of a multi-frequency ESR system with high sensitivity 2006/12
• 1P164 Analysis of Multi-Frequency and High-Field EPR Spectra for Powder and Single-Crystal of Mn(III)Mb with Integer Spin (S=2)(5. Heme protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006) 2006/10
• S = 2スピン系ヘムタンパク質の電子常磁性共鳴（EPR）による解析 ― Mn3+置換ミオグロビンの場合 ― 2003/04

Books

• ヘムタンパク質の科学、第5章 測定解析技術・第3節 "磁気共鳴EPRでヘムタンパク質の何をみるか？―活性中心の電子状態とミクロ分子環境―";　2022/05
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 生命金属ダイナミクス～生体内における金属の挙動と制御～;　2021/01
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  

Original Articles

• Quick and Spontaneous Transformation between [3Fe-4S] and [4Fe-4S] Iron-Sulfur Clusters in the tRNA-Thiolation Enzyme TtuA;　2023/01
  ANNOUNCEMENT INFO.;　International Jouranl of Molecular Science, in press
  AUTHOR;　Masato Ishizaka, Mihghao Chen, Shun Narai, Yoshikazu Tanaka, Toyoyuki Ose, Masaki Horitani*, and Min Yao*
  
• Intermolecular Electron Transfer from Biopterin to FeII-O2 Complex in Nitric Oxide Synthases Occurs at very Different Rates Between Bacterial and Mammalian Enzymes: Direct Observation of a Catalytically Active Intermediate;　2023/01
  ANNOUNCEMENT INFO.;　Journal of Inorganic Biochemistry, 238, 112035
  AUTHOR;　Kazuo Kobayashi*, Yuko Tsutsui Ito, Yuri Kasu, Masaki Horitani, and Takahiro Kozawa
  
• Trapping of Mono-Nitrosyl Non-Heme Intermediate of Nitric Oxide Reductase by Cryo-photolysis of Caged Nitric Oxide;　2023/00
  ANNOUNCEMENT INFO.;　Journal Physical Chemistry B, in press
  AUTHOR;　Hanae Takeda, Kanji Shimba, Masaki Horitani, Tetsunari Kimura, Takashi Nomura, Minoru Kubo, Yoshitsugu Shiro*, and Takehiko Tosha*
  
• Heme Protein Identified from Scaly-Foot Gastropod can Synthesize Pyrite (FeS2) Nanoparticles;　2023/00
  ANNOUNCEMENT INFO.;　Acta Biomaterialia, in press
  AUTHOR;　Tatsuya Yamashita, Hiroki Matsuda, Kyohei Koizumi, Logu Thirumalaisamy, Myeongok Kim, Lumi Negishi, Hitoshi Kurumizaka, Yoriko Tominaga, Yoshihiro Takagi, Ken Takai, Taiga Okumura, Hidekazu Katayama, Masaki Horitani, Nazmul Ahsan, Yoshitaka Okada, Koji Nagata, Yohey Suzuki, Michio Suzuki*
  
• The molecular mechanism of phytosphingosine binding to FFAR4/GPR120 differs from that of other fatty acids;　2021/11
  ANNOUNCEMENT INFO.;　FEBS open bio, 11, 11, 3081-3089
  AUTHOR;　Tomotaka Nagasawa, Masaki Horitani, Shin-ichi Kawaguchi, Shigeki Higashiyama, Yoichiro Hama, Susumu Mitsutake*
  
• Biochemical Properties of CumA Multicopper Oxidase from Plant Pathogen, Pseudomonas syringae;　2021/07
  ANNOUNCEMENT INFO.;　Bioscience, Biotechnology, and Biochemistry, 85, 9, 1995-2002
  AUTHOR;　Konoan Ishida, Yuya Tsukamoto, Masaki Horitani*, Tomohisa Ogawa, and Yoshikazu Tanaka*
  
• Timing of NO Binding and Protonation in the Catalytic Reaction of Bacterial Nitric Oxide Reductase as Established by Time-Resolved Spectroscopy;　2020/07
  ANNOUNCEMENT INFO.;　Bulletin of the Chemical Society of Japan, 93, 7, 825-833
  AUTHOR;　Hanae Takeda, Tetsunari Kimura, Takashi Nomura, Masaki Horitani, Azusa Yokota, Akiko Matsubayashi, Shoko Ishii, Yoshitsugu Shiro, Minoru Kubo*, Takehiko Tosha*
  
• The [4Fe-4S] Cluster of Sulfurtransferase TtuA Desulfurizes TtuB during tRNA Modification in Thermus Thermophilus;　2020/04
  ANNOUNCEMENT INFO.;　Communications Biology, 3, 168, 1-9
  AUTHOR;　Minghao Chen, Masato Ishizaka, Shun Narai, Masaki Horitani, Naoki Shigi, Min Yao*, and Yoshikazu Tanaka*
  
• X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy Reveal Active Site Rearrangement of Cold-Adapted Inorganic Pyrophosphatase;　2020/03
  ANNOUNCEMENT INFO.;　Scientific Reports, 10, 4368
  AUTHOR;　Masaki Horitani*, Kazuki Kusubayashi, Kyoka Oshima, Akane Yato, Hiroshi Sugimoto, and Keiichi Watanabe
  
• An Ancient Type of MnmA Protein is an Iron-Sulfur Cluster-Dependent Sulfurtransferase for tRNA Anticodons;　2019/12
  ANNOUNCEMENT INFO.;　RNA, 25, 240-250
  AUTHOR;　Naoki Shigi*, Masaki Horitani, Kenjyo Miyauchi, Tsutomu Suzuki, and Misao Kuroki
  
• Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme;　2017/08
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 139, 34, 11803-11813
  AUTHOR;　Krista A. Shisler, Rachel U. Hutcheson, Masaki Horitani, Kaitlin S. Duschene, Adam V. Crain, Amanda S. Byer, Eric M. Shepard, Ashley Rasmussen, Jian Yang, William E. Broderick, Jessica L. Vey, Catherine L. Drennan, Brian M. Hoffman, and Joan B. Broderick
  
• ENDOR Characterization of an Iron-Alkene Complex Provides Insight into a Corresponding Organometallic Intermediate of Nitrogenase;　2017/06
  ANNOUNCEMENT INFO.;　Chemical Science, 8, 5941-5948
  AUTHOR;　Masaki Horitani, Katarzyna Grubel, Sean F. McWilliams, Bryan D. Stubbert, Brandon Q. Mercado, Ying Yu, Prabhuodeyara M. Gurubasavaraj, Nicholas S. Lees, Patrick L. Holland, and Brian M. Hoffman
  
• Substrate-Dependent Cleavage Site Selection by Unconventional Radical S-Adenosylmethionine Enzymes in Diphthamide Biosynthesis;　2017/04
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 139, 16, 5680-5683
  AUTHOR;　Dong, Min; Horitani, Masaki; Horitani, Masaki; Dzikovski, Boris; Freed, Jack H.; Ealick, Steven E.; Hoffman, Brian M.; Lin, Hening
  
• ¹³C ENDOR spectroscopy of lipoxygenase-substrate complexes reveals the structural basis for C-H activation by tunneling;　2017/02
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 139, 5, 1984-1997
  AUTHOR;　Horitani, Masaki; Offenbacher, Adam R.; Marcus Carr, Cody A.; Yu, Tao; Hoeke, Veronika; Cutsail, George E.; Hammes-Schiffer, Sharon; Klinman, Judith P.; Hoffman, Brian M.
  
• Negative cooperativity in the nitrogenase Fe protein electron delivery cycle;　2016/10
  ANNOUNCEMENT INFO.;　Proceedings of the National Academy of Sciences of the United States of America, 113, 40, E5783-E5791
  AUTHOR;　Danyal, Karamatullah; Shaw, Sudipta; Page, Taylor R.; Duval, Simon; Horitani, Masaki; Marts, Amy R.; Lukoyanov, Dmitriy; Dean, Dennis R.; Raugei, Simone; Hoffman, Brian M.; Seefeldt, Lance C.; Antony, Edwin
  
• Organometallic Complex Formed by an Unconventional Radical S-Adenosylmethionine Enzyme;　2016/08
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 138, 31, 9755-9758
  AUTHOR;　Dong, Min; Horitani, Masaki; Dzikovski, Boris; Pandelia, Maria Eirini; Krebs, Carsten; Freed, Jack H.; Hoffman, Brian M.; Lin, Hening; Lin, Hening
  
• Metalloproteins: Radical SAM catalysis via an organometallic intermediate with an Fe-[5′-C]-deoxyadenosyl bond;　2016/05
  ANNOUNCEMENT INFO.;　Science, 352, 6287, 822-825
  AUTHOR;　Horitani, Masaki; Shisler, Krista; Broderick, William E.; Hutcheson, Rachel U.; Duschene, Kaitlin S.; Marts, Amy R.; Hoffman, Brian M.; Broderick, Joan B.
  
• Investigating the position of the hairpin loop in New Delhi metallo-β-lactamase, NDM-1, during catalysis and inhibitor binding;　2016/03
  ANNOUNCEMENT INFO.;　Journal of Inorganic Biochemistry, 156, 35-39
  AUTHOR;　Aitha, Mahesh; Moller, Abraham J.; Sahu, Indra D.; Horitani, Masaki; Tierney, David L.; Crowder, Michael W.
  
• Why Nature Uses Radical SAM Enzymes so Widely: Electron Nuclear Double Resonance Studies of Lysine 2,3-Aminomutase Show the 5′-dAdo• "free Radical" Is Never Free;　2015/06
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 137, 22, 7111-7121
  AUTHOR;　Horitani, Masaki; Byer, Amanda S.; Shisler, Krista A.; Chandra, Tilak; Broderick, Joan B.; Hoffman, Brian M.
  
• Characterization of the Fe-H bond in a three-coordinate terminal hydride complex of iron(I);　2012/04
  ANNOUNCEMENT INFO.;　Angewandte Chemie - International Edition, 51, 15, 3658-3662
  AUTHOR;　Chiang, Karen P.; Scarborough, Christopher C.; Horitani, Masaki; Lees, Nicholas S.; Ding, Keying; Dugan, Thomas R.; Brennessel, William W.; Bill, Eckhard; Hoffman, Brian M.; Holland, Patrick L.
  
• Low-lying electronic states of the ferrous high-spin (S = 2) heme in deoxy-Mb and deoxy-Hb studied by highly-sensitive multi-frequency EPR;　2011/12
  ANNOUNCEMENT INFO.;　Journal of Inorganic Biochemistry, 105, 12, 1596-1602
  AUTHOR;　Hori, Hiroshi; Yashiro, Haruhiko; Yashiro, Haruhiko; Ninomiya, Kenta; Horitani, Masaki; Kida, Takanori; Hagiwara, Masayuki
  
• Resonance Raman study on the oxygenated and the ferryl-oxo species of indoleamine 2,3-dioxygenase during catalytic turnover;　2011/12
  ANNOUNCEMENT INFO.;　Faraday Discussions, 148, 239-247
  AUTHOR;　Yanagisawa, Sachiko; Horitani, Masaki; Sugimoto, Hiroshi; Shiro, Yoshitsugu; Okada, Norihiro; Ogura, Takashi
  
• Resonance Raman identification of a FeIV=O type reaction intermediate during indoleamine 2, 3-dioxygenase reaction;　2010/12
  ANNOUNCEMENT INFO.;　AIP Conference Proceedings, 1267, 892-893
  AUTHOR;　Ogura, Takashi; Yanagisawa, Sachiko; Okada, Norihiro; Horitani, Masaki; Sugimoto, Hiroshi; Shiro, Yoshitsugu; Appelman, Evan H.
  
• Identification of the Fe-O 2 and the Fe=O heme species for indoleamine 2,3-dioxygenase during catalytic turnover;　2010/02
  ANNOUNCEMENT INFO.;　Chemistry Letters, 39, 1, 36-37
  AUTHOR;　Yanagisawa, Sachiko; Yotsuya, Keiko; Hashiwaki, Yumi; Horitani, Masaki; Sugimoto, Hiroshi; Shiro, Yoshitsugu; Appelman, Evan H.; Ogura, Takashi
  
• Multi-frequency and high-field EPR study of manganese(III) protoporphyrin IX reconstituted myoglobin with an S = 2 integer electron spin;　2008/04
  ANNOUNCEMENT INFO.;　Journal of Inorganic Biochemistry, 102, 4, 781-788
  AUTHOR;　Horitani, Masaki; Yashiro, Haruhiko; Hagiwara, Masayuki; Hori, Hiroshi
  
• Development of a multi-frequency ESR system with high sensitivity;　2006/12
  ANNOUNCEMENT INFO.;　Journal of Physics: Conference Series, 51, 1, 576-579
  AUTHOR;　Yashiro, H.; Kashiwagi, T.; Horitani, M.; Hobo, F.; Hori, H.; Hagiwara, M.
  

Material, Commentary, Editorials, Research Report, A Comprehensive Journal Articles

• 鉄硫黄クラスターが関与するtRNAチオ化修飾酵素の詳細な反応機構解明を目指した研究;　2019/11
  ANNOUNCEMENT INFO.;　Photon Factory News, 37, 20-24
  AUTHOR;　
  
• Trapping of the Reaction Intermediates and Analysis of Enzymatic Catalytic reaction on Radical SAM Enzymes;　2017/08
  ANNOUNCEMENT INFO.;　SEIBUTSU BUTSURI, 57, 4, 202-204
  AUTHOR;　Masaki Horitani
  
• 先端磁気共鳴法による金属タンパク質研究;　2017/04
  ANNOUNCEMENT INFO.;　, 15, 20-26
  AUTHOR;　
  
• 多周波数・強磁場EPR(MFEPR)法を用いた整数スピン系金属タンパク質研究の新たな展開 ～　Mn3+ポルフィリン置換ミオグロビン(S = 2)　～;　2007/04
  ANNOUNCEMENT INFO.;　, 平成１９年度, 76-76
  AUTHOR;　
  
• S = 2スピン系ヘムタンパク質の電子常磁性共鳴（EPR）による解析 ― Mn3+置換ミオグロビンの場合 ―;　2003/04
  ANNOUNCEMENT INFO.;　, 平成15年, 35-38
  AUTHOR;　
  

General Lectures

• スピンラベル-ESR法を利用した酵素の構造柔軟性の実測;　2022/12
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• ヘムトランスポーターの輸送機構解明へ向けた新たな反応中間体捕捉法の開発;　2022/12
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 急速凍結-電子常磁性共鳴（EPR）法を用いた金属酵素活性中心における構造変化の温度依存性;　2022/12
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• ヘム駆動型フェロミクスプローブの反応機構解明;　2022/12
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• EPR Spectroscopy Reveals the Differences of Active Site Structure for Di-Mn Enzymes from Mesophillic and Psychrophillic Bacteria;　2022/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• X-ray Crystallography and Spin-Labeling ESR Reveal Cold Adaptation and High Thermal Stability Mechanism of Cold-Adapted Glucokinase;　2022/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 先端X線回折法によるグルコキナーゼの動的構造-機能相関の解明;　2022/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 反応中間体捕捉から解き明かすミモシン合成酵素の2機能性獲得機構;　2022/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 先端ESR法と生命科学研究への潮流;　2022/09
  ANNOUNCEMENT INFO.;　令和4年度生命金属科学夏の合宿
  AUTHOR;　
  
• X線結晶構造解析による可溶性Family II無機ピロフォスファターゼの触媒活性及び構造ダイナミクス機構の解明;　2022/09
  ANNOUNCEMENT INFO.;　令和4年度生命金属科学夏の合宿
  AUTHOR;　
  
• 電子スピン共鳴法で”観る”金属酵素活性部位の温度による微細構造変化;　2022/07
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• スピンラベル-ESR法を利用した酵素の構造柔軟性の実測;　2022/07
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 生命金属動態の解明に向けた光照射EPR技術による新しい酵素反応反応機構法の開発;　2022/05
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Crystal Structure of Soluble Family II Inorganic Pyrophosphatase Revealed the Mechanism of Catalysis and Structure Dynamics;　2021/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Kantaro Sakamoto, Hiroshi Sugimoto, Masaki Horitani
  
• Structural Insights into Catalytic Reaction Mechanism for bifunctional enzyme, Mimosine Synthase, from Leucaena leucocephala;　2021/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Masaki Horitani, Risa Maeda, Shigeki Oogai, Masakazu Fukuta, Hirosuke Oku, Hiroshi Sugimoto
  
• X-ray crystallography and spin-labeling ESR reveal cold adaptation and high thermal stability mechanisms of cold-adapted glucokinase;　2021/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Akane Yato, Rio Asaka, Hiroshi Sugimoto, Keiichi Watanabe, Masaki Horitani
  
• Reaction mechanism of tRNA thiolation revealed by correlation analysis between the structure of Fe-S clusters and the enzymatic activity;　2021/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Masato Ishizaka, Minghao Chen, Shun Narai, Masaki Horitani, Yoshikazu Tanaka, Min Yao
  
• 急速凍結XAFS法による低温適応無機ピロフォスファターゼの温度特性機構の解明;　2021/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Analysis on Cold Adaptation Mechanism of Metalloenzyme by X-ray Crystallography and EPR Spectroscopy Combined with Rapid Freeze-Quench;　2021/08
  ANNOUNCEMENT INFO.;　22nd International Society of Magnetic Resonance, 9th Asia-Pacific NMR Symposium (APNMR9), 60th Annual Meeting of the Nuclear Magnetic Resonance Society of Japan (2021), 60th Annual Meeting of the Society of Electron Spin Science and Technology (SEST2021)
  AUTHOR;　Masaki Horitani, Yuri Kasu, Hiroshi Sugimoto, Keiichi Watanabe
  
• X-ray Crystallography and Site-Directed Spin-Labeling ESR Reveal Cold Adaptation and High Thermal Stability Mechanisms of Cold-Adapted Glucokinase;　2021/08
  ANNOUNCEMENT INFO.;　22nd International Society of Magnetic Resonance, 9th Asia-Pacific NMR Symposium (APNMR9), 60th Annual Meeting of the Nuclear Magnetic Resonance Society of Japan (2021), 60th Annual Meeting of the Society of Electron Spin Science and Technology (SEST2021)
  AUTHOR;　Akane Yato, Rio Asaka, Hiroshi Sugimoto, Keiichi Watanabe, Masaki Horitani
  
• 動的・静的構造解析による南極産好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明;　2021/06
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 急速凍結XAFS分光法による低温適応無機ピロフォスファターゼの金属活性中心の局所構造解析;　2021/06
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• X線結晶構造解析法による低温適応無機ピロフォスファターゼの触媒活性機構の解明;　2021/06
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 急速凍結EPR法およびX線結晶構造解析法による好冷細菌由来無機ピロフォスファターゼの低温適応機構の解明;　2021/06
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 高速混合凍結EPR法による低温適応酵素の低温適応機構解析;　2020/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 整数スピン系ヘムタンパク質研究に応用可能な先端テラヘルツESR装置開発;　2020/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明;　2020/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• X線結晶構造解析および高速混合凍結・電子常磁性共鳴法による南極由来低温適応酵素の静的・動的構造解析;　2020/10
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 南極産好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明;　2020/10
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 南極産好冷細菌由来ピロフォスファターゼのX線結晶構造解析による低温適応機構の解明;　2020/10
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Cold Adaptation and High Thermal Stability Mechanism of Glucokinase from psychrophillic bacteria are revealed by spin-labeling ESR;　2020/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 好冷細菌由来無機ピロフォスファターゼの低温適応機構・熱安定性機構;　2019/12
  ANNOUNCEMENT INFO.;　日本生体エネルギー研究会　第45回討論会
  AUTHOR;　堀谷　正樹, 杉本　宏, 渡邊　啓一
  
• 好冷細菌由来グルコキナーゼの低温適応・高熱安定性機構の解明;　2019/12
  ANNOUNCEMENT INFO.;　日本生体エネルギー研究会　第45回討論会
  AUTHOR;　矢垰　紅音, 浅香　里緒, 渡邊　啓一, 堀谷　正樹
  
• 好冷細菌由来無機ピロフォスファターゼの低温適応機構;　2019/11
  ANNOUNCEMENT INFO.;　第58回NMR討論会・SEST2019連合大会
  AUTHOR;　堀谷　正樹, 楠林　和貴, 大嶋　杏佳, 杉本　宏, 渡邉　啓一
  
• 先端テラヘルツESR装置開発によるヘムタンパク質研究の新展開;　2019/07
  ANNOUNCEMENT INFO.;　九重分子科学セミナー2019
  AUTHOR;　二宮　春菜, 岡本　翔, 西川　佳那, 大久保　晋, 大道　英二, 藤井　浩, 太田　仁, 堀谷　正樹
  
• Electron Paramagnetic Resonance Reveals Cold Adaption Mechanism of the Psychrophilic Metalloenzyme;　2019/06
  ANNOUNCEMENT INFO.;　第19回日本蛋白質科学会年会、第71回日本細胞生物学会大会　合同年次大会
  AUTHOR;　Masaki Horitani
  
• Development of Advanced THz ESR Spectroscopy for Heme Proteins with Integer Spin System;　2019/06
  ANNOUNCEMENT INFO.;　Awaji Island Conference on Electron Spin Science & Technology
  AUTHOR;　Haruna Ninomiya, Tsubasa Okamoto, Kana Nishikawa, Susumu Okubo, Eiji Omichi, Hiroshi Fujii, Hitoshi Ohta, Masaki Horitani
  
• Structural Analysis on di-Mn ion site for Shewanella inorganic pyrophosphatase by EPR;　2018/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 電子常磁性共鳴法による低温適応酵素無機ピロフォスファターゼの複核Mnイオンサイトの構造解析;　2018/07
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• ENDOR 法によるタンパク質‐基質複合体精密構造解析　～酵素によるトンネリング機構解明へ～;　2017/11
  ANNOUNCEMENT INFO.;　第56回電子スピンサイエンス学会年会（SEST2017）
  AUTHOR;　
  
• Substrate Positioning for H-Atom Abstraction in Soybean Lipoxygenase by ENDOR Crystallography;　2017/06
  ANNOUNCEMENT INFO.;　The 17th Annual Meeting of the Protein Science Society of Japan
  AUTHOR;　Masaki Horitani, Adam R. Offenbacher, Cody A. Marcus Carr, Tao Yu, Veronika Heke, George E. Cutsail III, Sharon Hammes-Schiffer, Judith P. Klinman, Brian M. Hoffman
  
• 先端磁気共鳴法によるラジカルSAM酵素の反応機構;　2017/01
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 先端磁気共鳴法による金属タンパク質研究;　2016/11
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• ラジカルSAM酵素における有機金属反応中間体の観測;　2016/06
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Masaki Horitani、Krista Shisler, William E. Broderick, Rachel U. Hatcheson, Kaitlin S. Duschene, Amy R. Marts, Brian M. Hoffman, Joan B. Broderick
  
• ENDOR法によるラジカルSAM酵素の反応機構解明-なぜ生物はラジカルSAM酵素を広く利用するのか？-;　2015/06
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• New Approach to ENDOR Analysis for Manganese(II)-Substituted Soybean Lipoxygenase with A Non-Coordinated Substrate;　2014/11
  ANNOUNCEMENT INFO.;　Joint Conference of 9th Asia-Pacific EPR/ESR Society Symposium, 1st International EPR (ESR) Society Symposium, 53rd SEST Annual Meeting
  AUTHOR;　Masaki Horitani, George Cutsail III, Cody A. Marcus Carr, Adam R. Offenbacher, Judith P. Klinman, and Brian M. Hoffman
  
• The Structural Analysis for Reaction Intermediate of Lysine 2,3-Aminomutase by ENDOR Spectroscopy;　2012/08
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  

Others

• 新規な酸素感受性tRNA硫黄化酵素の機能解析;　2018/12
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Elucidation of the tRNAsulfurationmechanism of TtuA involved inFe-S cluster;　2018/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Masato Ishizaka, Minghao Chen, Syun Narai, Masaki Horitani, Seiko Oka, YoshikzauTanaka, and Min Yao
  
• 低温適応酵素グルコキナーゼの低温機構解明;　2018/08
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 低温適応酵素グルコキナーゼの低温機構解明に向けて;　2017/11
  ANNOUNCEMENT INFO.;　, 第56回電子スピンサイエンス学会年会（SEST2017）
  AUTHOR;　
  
• 電子スピン共鳴法による低温適応無機ピロフォスファターゼ活性部位Mn2+イオンの電子状態解析;　2017/08
  ANNOUNCEMENT INFO.;　, 第41回蛋白質と酵素の構造と機能に関する九州シンポジウム
  AUTHOR;　
  
• 3P025 Taming the Reactive 5'-Deoxyadenosyl Radical by Enforcing van der Waals Contact with Substrate in Lysine 2,3-Aminomutase(01A. Protein: Structure,Poster);　2013/09
  ANNOUNCEMENT INFO.;　Biophysics, 53, 1
  AUTHOR;　Horitani Masaki;Shisler Krista;Byer Amanda;Broderick Joan B.;Hoffman Brian M.
  
• Letters from Abroad;　2013/07
  ANNOUNCEMENT INFO.;　Seibutsu Butsuri, 53, 4, 227228
  AUTHOR;　
  
• 2P103 Low Lying Electronic States of the Ferrous High-Spin (S=2) Heme in Deoxy-Hb Studied by High-Sensitive Multi-Frequency EPR(The 48th Annual Meeting of the Biophysical Society of Japan);　2010/08
  ANNOUNCEMENT INFO.;　Biophysics, 50, 2
  AUTHOR;　Hori Hiroshi;Ninomiya Kenta;Horitani Masaki;Yashiro Haruhiko;Hagiwara Masayuki
  
• 2P110 Elucidation of NO reduction reaction using Zn-substituted bacterial nitric oxide reductase(The 48th Annual Meeting of the Biophysical Society of Japan);　2010/08
  ANNOUNCEMENT INFO.;　Biophysics, 50, 2
  AUTHOR;　Okada Norihiro;Tosha Takehiko;Horitani Masaki;Shiro Yoshitsugu
  
• 1P-082 Role of the Heme Environmental Amino Acid Residues and Mechanism of the Substrate Inhibition in Human Indoleamine 2,3-Dioxygenase(Heme proteins, The 47th Annual Meeting of the Biophysical Society of Japan);　2009/09
  ANNOUNCEMENT INFO.;　Biophysics, 49, 1
  AUTHOR;　Horitani Masaki;Sugimoto Hiroshi;Kometani Erisa;Yamamoto Tatsuya;Shiro Yoshitsugu
  
• 3P-077 Detection of Two Oxygen-Isotope Sensitive Resonance Raman Bands for Human Indoleamine 2,3-Dioxygenase(Heme proteins,The 47th Annual Meeting of the Biophysical Society of Japan);　2009/09
  ANNOUNCEMENT INFO.;　Biophysics, 49, 1
  AUTHOR;　Yanagisawa Sachiko;Yotsuya Keiko;Hashiwaki Yumi;Horitani Masaki;Sugimoto Hiroshi;Shiro Yoshitsugu;Ogura Takashi
  
• 2SA1-03 High-field and Multi-frequency EPR study of metalloproteins with non-Kramers ions(2SA1 Electron Spin Resonance on Biophysics -The Next Generation-,The 47th Annual Meeting of the Biophysical Society of Japan);　2009
  ANNOUNCEMENT INFO.;　Seibutsu Butsuri, 49, 0
  AUTHOR;　Yashiro Haruhiko;Ninomiya Kenta;Horitani Masaki;Hori Hiroshi;Hagiwara Masayuki
  
• 1P-120 High-Field and Multi-Frequency EPR in Deoxyhemoglobin(The 46th Annual Meeting of the Biophysical Society of Japan);　2008/10
  ANNOUNCEMENT INFO.;　Biophysics, 48, 1, S39S40
  AUTHOR;　Yashiro Haruhiko;Horitani Masaki;Hagiwara Masayuki;Hori Hiroshi
  
• 23aQG-6 Development of a high-sensitive, multi-frequency ESR system utilizing a frequency standard and its application to metalloprotein with an integer spin;　2008/08
  ANNOUNCEMENT INFO.;　Meeting abstracts of the Physical Society of Japan, 63, 2
  AUTHOR;　Yashiro H.;Horitani M.;Kashiwagi T.;Hori H.;Hagiwara M.
  
• 21pPSB-62 Development of a High-Sensitive, Multi-Frequency EPR system with High Stability II;　2007/08
  ANNOUNCEMENT INFO.;　Meeting abstracts of the Physical Society of Japan, 62, 2
  AUTHOR;　Yashiro H.;Koda S.;Kashiwagi T.;Horitani M.;Hori H.;Hagiwara M.
  
• 1P164 Analysis of Multi-Frequency and High-Field EPR Spectra for Powder and Single-Crystal of Mn(III)Mb with Integer Spin (S=2)(5. Heme protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006);　2006/10
  ANNOUNCEMENT INFO.;　Biophysics, 46, 2
  AUTHOR;　Horitani Masaki;Yashiro Haruhiko;Hagiwara Masayuki;Hori Hiroshi
  
• 1P515 Development of a High-Sensitivitive, Multi-Frequency EPR system as a New Tool for Research on Metalloproteins with Integer Electron Spin(25. New methods and tools (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006);　2006/10
  ANNOUNCEMENT INFO.;　Biophysics, 46, 2
  AUTHOR;　Yashiro Haruhiko;Kashiwagi Takanari;Horitani Masaki;Hori Hiroshi;Hagiwara Masayuki
  
• 23aPS-121 Development of a High-Sensitive and Multi-Frequency ESR system with High Stability;　2006/08
  ANNOUNCEMENT INFO.;　Meeting abstracts of the Physical Society of Japan, 61, 2
  AUTHOR;　Yashiro H.;Kashiwagi T.;Horitani M.;Hori H.;Hagiwara M.
  
• 27pTH-3 High Magnetic Field EPR in a Metalloprotein with an Integer Spin; Mn^<3+> (S=2) Substituted Myoglobin;　2006/03
  ANNOUNCEMENT INFO.;　Meeting abstracts of the Physical Society of Japan, 61, 1
  AUTHOR;　Yashiro H.;Horitani M.;Hagiwara M.;Hori H.
  
• 3P082 Electron Paramagnetic Resonance Analysis of Mn^<3+>Mb with an S=2 Integer Spin;　2005
  ANNOUNCEMENT INFO.;　Seibutsu Butsuri, 45, 0
  AUTHOR;　Horitani M.;Yashio H.;Hagiwara M.;Hori H.
  
• 2P073 EPR analysis of hemoprotein with integar spin system (S=2) : Mn^<3+>-myoglobin;　2004
  ANNOUNCEMENT INFO.;　Seibutsu Butsuri, 44, 0
  AUTHOR;　Horitani M.;Murata T.;Hori H.
  

Invited Lecture, Special Lecture

• EPR Spectroscopy and X-ray Crystallography Revealed Rearrangements of Di-Mn Active Site by Substrate Binding in Inorganic Pyrophosphatase;　2023/11
  ANNOUNCEMENT INFO.;　10th Asian Biological Inorganic Chemistry Conference
  AUTHOR;　
  
• EPR Spectroscopy Reveals Active Site Rearrangements of Di-Mn Center in Inorganic Pyrophosphatase;　2022/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• 南極海由来金属酵素の酵素反応・低温適応機構;　2022/09
  ANNOUNCEMENT INFO.;　日本農芸化学会2022年度西日本支部大会
  AUTHOR;　
  
• X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy Reveal Active Site Rearrangement of Cold Adaptation Enzyme;　2021/12
  ANNOUNCEMENT INFO.;　The Internatinal Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  AUTHOR;　Masaki Horitani, Hiroshi Sugimoto, Keiichi Watanabe
  
• Advanced High Field and THz ESR Spectroscopic Studies on Heme Proteins with Integer Spin System;　2021/03
  ANNOUNCEMENT INFO.;　The 8th International Workshop on Far-Infrared Technologies
  AUTHOR;　Masaki Horitani, Haruna Ninomiya, Hideyuki Takahashi, Tsubasa Okamoto, Kana Nishikawa, Susumu Okubo, Eiichi Omichi, Hitoshi Ohta
  
• X-ray Crystallography and EPR Spectroscopy Reveal Active Site Rearrangement of Cold-Adapted Inorganic Pyrophosphatase;　2020/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Masaki Horitani, Hiroshi Sugimoto, Keiichi Watanabe
  
• ラジカルSAM酵素におけるラジカル反応機構～自然界はどのようにしてラジカルを安全に利用しているか～;　2019/09
  ANNOUNCEMENT INFO.;　2019年度第一回水和ナノ構造研究会
  AUTHOR;　堀谷　正樹
  
• Cold Adaption Inorganic Pyrophosphatase from Shewanella sp. AS-11 was Investigated the Reaction Mechanism by X-ray Crystallography and Electron Paramagnetic Resonance Spectroscopy;　2019/08
  ANNOUNCEMENT INFO.;　International Congress on Pure & Applied Chemistry (ICPAC) Yangon 2019
  AUTHOR;　Masaki Horitani, Hiroshi Sugimoto, Keiichi Watanabe
  
• EPR Spectroscopy Reveals the Active Site Rearrangement of Di-Mn2+ Center by Substrate Binding in Inorganic Pyrophosphatase from Shewanella sp. AS-11;　2019/06
  ANNOUNCEMENT INFO.;　Awaji Island Conference on Electron Spin Science & Technology
  AUTHOR;　Masaki Horitani, Hiroshi Sugimoto, Keiichi Watanabe
  
• Electron Paramagnetic Resonance Spectroscopy Investigates Di-Manganese(II) Active Site of Inorganic Pyrophosphate from Shewanella sp. AS-11;　2018/10
  ANNOUNCEMENT INFO.;　19th Malaysian International Chemistry Congress and International Congress on Pure & Applied Chemistry Langkawi 2018
  AUTHOR;　Masaki Horitani
  
• EPR Studies Reveal Mn(II)-Mn(II) Distance in the Active Site of Inorganic Pyrophosphatase from Shewanella sp. AS-11;　2018/09
  ANNOUNCEMENT INFO.;　The Third Joint Conference of the Asia-Pacific EPR/ESR Society and The International EPR (ESR) Society
  AUTHOR;　Masaki Horitani
  
• ラジカルSAM酵素はなぜ自然界に幅広く受け入れられてきたか～先端磁気共鳴法による酵素反応解析～;　2017/09
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Substrate Positioning of Soybean Lipoxygenase For H-Atom Abstraction by ENDOR 'Crystrallography';　2017/09
  ANNOUNCEMENT INFO.;　The 55th Annual Meeting of the Biophysical Society of Japan
  AUTHOR;　Masaki Horitani
  
• 生物学的プロトントンネリング機構解明へ～新規ENDOR解析法によるタンパク質構造精密解析;　2017/07
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Masaki Horitani
  
• New ENDOR Analysis Reveals the Structural Basis for C-H Activation by Biological Tunneling in Soybean Lipoxygenase;　2017/06
  ANNOUNCEMENT INFO.;　The 5th Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Science Oriented Applications (AWEST)
  AUTHOR;　Masaki Horitani, Adam R. Offenbacher, Cody A. Marcus Carr, Tao Yu, Veronika Heke, George E. Cutsail III, Sharon Hammes-Schiffer, Judith P. Klinman, Brian M. Hoffman
  
• ENDOR reveals how radical SAM enzymes tame highly reactive radical;　2016/11
  ANNOUNCEMENT INFO.;　Molecular Photoscience Research Center International Workshop “Novel Magnetic Resonance Techniques in Millimeter and Terahertz Waves and their Applications to Bioscience” (MR-THz2016)
  AUTHOR;　Masaki Horitani, Krista Shisler, William E. Broderick, Rachel U. Hatcheson, Kaitlin S. Duschene, Amy R. Marts, Brian M. Hoffman, Joan B. Broderick
  
• Radical SAM Catalysis via an Organometallic Intermediate with an Fe-[5’C-Deoxyadenosyl] Bond;　2016/06
  ANNOUNCEMENT INFO.;　The 4th Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Science Oriented Applications (AWEST)
  AUTHOR;　Masaki Horitani, Krista Shisler, William E. Broderick, Rachel U. Hatcheson, Kaitlin S. Duschene, Amy R. Marts, Brian M. Hoffman, Joan B. Broderick
  
• Why Nature Uses Radical SAM Enzyme Superfamily so Widely;　2015/12
  ANNOUNCEMENT INFO.;　BMB2015
  AUTHOR;　Masaki Horitani, Amanda S. Byer, Krista A. Shisler, Tilak Chandra, Joan B. Broderick, and Brian M. Hoffman
  
• 「留学のすゝめ2015」UAJWによる日本科学の推進;　2015/12
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Why Nature Uses Radical SAM Enzymes so Widely ~ENDOR Studies on Lysine 2,3-Aminomutase Show the 5’-dAdo• ‘Free Radical’ is Never Free~;　2015/06
  ANNOUNCEMENT INFO.;　The 3rd Awaji International Workshop on Electron Spin Science & Technology: Biological and Materials Science Oriented Applications (AWEST)
  AUTHOR;　Masaki Horitani, Amanda S. Byer, Krista A. Shisler, Tilak Chandra, Joan B. Broderick, and Brian M. Hoffman