NameToyoaki ANAI
DepartmentFaculty of Agriculture
Job Title Degree Obtained
E-mailanaicc.saga-u.ac.jp
Homepage

Detailed Information

Research Field/Keywords for Research Field

  • soybean, mutant, genome analysis, transgenic plant

Education

  • 1989/03, Graduated
  • 1991/03, Master Course, Completed
  • 1994/03, Doctor Course, Completed

Employment Experience

  • 1994/04 - 1997/03 Research Associate
  • 1997/04 - 2007/03 Associate Professor, Faculty of Agriculture, Saga University
  • 2000/04 - 2003/10 Associate Professor
  • 2007/04 - 2013/05 Associate Professor, Applied Biological Sciences, Faculty of Agriculture, Saga University
  • 2007/04 - 9999/99 Associate Professor
  • 2013/06 -  *  Professor, Applied Biological Sciences, Faculty of Agriculture, Saga University
  • 2013/10 - 9999/99 Professor

Field of Specialization

  • Science in genetics and breeding, Crop production science, Applied molecular and cellular biology, Applied biochemistry, Food science

Awards

  • 日本育種学会賞 (2019/03)
  • 日本育種学会論文賞 (2011/04)
  • 佐賀県科学技術奨励賞 (2002/01)

Research Topics and Results

  • Identification of corresponding genes for three low-a-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed. 2005
  • Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene 2003

Books

  • Mutant-based reverse genetics for functional genomics of non-model crops; 2016/03
    ANNOUNCEMENT INFO.; Advances in Plant Breeding Strategies, 1: Breeding, Biotechnology and Molecular Tools, 473-487
    AUTHOR; Toyoaki Anai
  • Positional cloning of the responsible genes for maturity loci E1, E2 and E3 in soybean; 2011/11
    ANNOUNCEMENT INFO.; Soybean - Genetics and Novel Techniques for Yield Enhancement, Dora Krezhova (Ed.), InTech, Available from: http://www.intechopen.com/articles/show/title/positional-cloning-of-the-responsible-genes-for-maturity-loci-e1-e2-and-e3-in-soybean
    AUTHOR; Kyuya Harada, Satoshi Watanabe, Xia Zhengjun, Yasutaka Tsubokura, Naoki Yamanaka, Toyoaki Anai
  • 植物生理化学研究と遺伝子解析法; 2011/10
    ANNOUNCEMENT INFO.; , 306-322
    AUTHOR; 
  • Mutation Breeding of Soybean in Japan; 2011/01
    ANNOUNCEMENT INFO.; Breeding of Pulse Crops (Kalyani Publishers), 61-93
    AUTHOR; Hitoshi Nakagawa, Toyoaki Anai, Akinori Okabe, Koji Takahashi, Makita Hajika and Yutaka Takagi
  • Development of novel fatty acid composition in soybean oil through induced mutation; 2011
    ANNOUNCEMENT INFO.; , 437-444
    AUTHOR; Takagi, Y., Anai, T., Nakagawa, H.
  • 7.1 生理活性物質(分子・構造・作用・受容体・分布・容量など); 2002/09
    ANNOUNCEMENT INFO.; , 1591-1601
    AUTHOR; 
  • 植物のGタンパク質と細胞内情報伝達; 1995/02
    ANNOUNCEMENT INFO.; , 108-114
    AUTHOR; 

Original Articles

  • SEN1 gene from Lotus japonicus MG20 improves nitrogen fixation and plant growth.; 2020/10
    ANNOUNCEMENT INFO.; Soil Science and Plant Nutrition, 66, 864-869
    AUTHOR; Y. Nishida ,R. Hiraoka , S. Kawano , N. Suganuma , S. Sato , S. Watanabe , T. Anai , S. Arima , A. Tominaga, A. Suzuki
  • Characterization of an Intermediate Filament Protein from the Platyhelminth, Dugesia japonica; 2020/05
    ANNOUNCEMENT INFO.; Protein and Peptide Letters, 27, 5, 432-446
    AUTHOR; A. Yamamoto, K-I. Matsunaga, T. Anai, H. Kawano, T. Ueda, T. Matsumoto, S. Ando
  • Identification of a cytochrome P450 hydroxylase, CYP81E22, as a causative gene for the high sensitivity of soybean to herbicide bentazon; 2020/03
    ANNOUNCEMENT INFO.; Theoretical and Applied Genetics, 133, 2105-2115
    AUTHOR; S. Kato, Y. Yokota, R. Suzuki, Y. Fujisawa, T. Sayama, A. Kaga, T. Anai, K. Komatsu, N. Oki, A. Kikuchi, M. Ishimoto
  • Single-base deletion in GmCHR5 increases the genistein-to-daidzein ratio in soybean seed; 2020/03
    ANNOUNCEMENT INFO.; Breeding Science, 70, 265-276
    AUTHOR; Md. A. R. Sarkar, W. Otsu, A. Suzuki, F. Hashimoto, T. Anai, S. Watanabe
  • An Agrobacterium-mediated transformation via organogenesis regeneration of a facultative CAM plant, the common ice plant Mesembryanthemum crystallinum L; 2020/03
    ANNOUNCEMENT INFO.; Plant Production Science, 23, 3, 343-349
    AUTHOR; S. Agarie, M. Umemoto, H. Sunagawa, T. Anai, J. C. Cushman
  • Molecular characterization of high stearic acid soybean mutants and post-transcriptional control of GmSACPD genes in the mutant with a single nucleotide deletion; 2020/03
    ANNOUNCEMENT INFO.; Plant Gene, 21, 100207
    AUTHOR; T. Hoshino, N. Iijima, M. Hata, A. Watanabe, T. Kawakami, T. Anai
  • Identification of novel MYB transcription factors involved in the isoflavone biosynthetic pathway by using the combination screening system with agroinfiltration and hairy root transformation; 2019/12
    ANNOUNCEMENT INFO.; Plant Biotechnology, 36, 241-251
    AUTHOR; Md. A. R. Sarkar, S. Watanabe, A. Suzuki, F. Hashimoto, T. Anai
  • Domesticating Vigna stipulacea: a Potential Legume Crop with Broad Resistance to Biotic Stresses; 2019/12
    ANNOUNCEMENT INFO.; Frontiers in Plant Science, 10, 1607
    AUTHOR; Y. Takahashi, H. Sakai, Y. Yoshitsu, C. Muto, T. Anai, M. Pandiyan, N. Senthil, N. Tomooka, K. Naito
  • Identification and characterization of a major QTL underlying soybean isoflavone malonylglycitin content; 2019/09
    ANNOUNCEMENT INFO.; Breeding Science, 69, 564-572
    AUTHOR; S. Watanabe, R. Yamada, H. Kanetake, A. Kaga, T. Anai
  • Soybean antiviral immunity conferred by dsRNasetargets the viral replication complex; 2019/09
    ANNOUNCEMENT INFO.; Nature Communications, 10, 4033
    AUTHOR; K. Ishibashi, M. Saruta, T. Shimizu, M. Shu, T. Anai, K. Komatsu, N. Yamada, Y. Katayose Y, M. Ishikawa, M. Ishimoto, A. Kaga
  • Isolation and characterization of induced mutants in the gene associated with seed cadmium accumulation in soybean; 2019/07
    ANNOUNCEMENT INFO.; Breeding Science, 69, 345–351
    AUTHOR; Kaori Hirata, Kyoko Takagi, Tetsuya Yamada, Takashi Sayama, Toyoaki Anai, Akio Kikuchi and Masao Ishimoto
  • High-Throughput Screening and Characterization of a High-Density Soybean Mutant Library Elucidate the Biosynthesis Pathway of Triterpenoid Saponins.; 2019/02
    ANNOUNCEMENT INFO.; Plant and Cell Physiology, 60, 5, 1082-1097
    AUTHOR; P. Krishnamurthy, Y. Fujisawa, Y. Takahashi, H. Abe, K. Yamane, K. Mukaiyama, H.-R. Son, S. Hiraga, A. Kaga, T. Anai, C. Tsukamoto, M. Ishimoto
  • Selection criteria for SNP loci to maximize robustness of high-resolution melting analysis for plant breeding.; 2018/10
    ANNOUNCEMENT INFO.; Breeding Science, 68, 4, 488-498
    AUTHOR; Y. Yamagata, A. Yoshimura, T. Anai, S. Watanabe
  • Genetic and functional characterization of Sg-4 glycosyltransferase involved in the formation of sugar chain structure at the C-3 position of soybean saponins.; 2018/09
    ANNOUNCEMENT INFO.; Phytochemistry, 156, 96-105
    AUTHOR; K. Takagi, R. Yano, S. Tochigi, Y. Fujisawa, H. Tsuchinaga, Y. Takahashi, Y. Takada, A. Kaga, T. Anai, C. Tsukamoto, H. Seki, T. Muranaka, M. Ishimoto
  • High-resolution mapping and characterization of xa42, a resistance gene against multiple Xanthomonas oryzae pv. oryzae races in rice (Oryza sativa L.).; 2018/05
    ANNOUNCEMENT INFO.; Breeding Science, 68, 188-199
    AUTHOR; C. Busungu, S. Taura, J.-I. Sakagami, T. Anai, K. Ichitani
  • Cytosolic Cysteine Synthase Switch Cysteine and Mimosine Production in Leucaena leucocephala; 2018/04
    ANNOUNCEMENT INFO.; Applied Biochemistry and Biotechnology, 186, 613–632
    AUTHOR; Md. H.-U.-Rashid, H. Iwasaki, S. Parveen, S. Oogai, M. Fukuta, Md. A. Hossain, T. Anai, H. Oku
  • Isolation and Characterization of the Soybean Sg-3 Gene that is Involved in Genetic Variation in Sugar Chain Composition at the C-3 Position in Soyasaponins.; 2018/04
    ANNOUNCEMENT INFO.; Plant and Cell Physiology, 59, 797-810
    AUTHOR; R. Yano, K. Takagi, S. Tochigi, Y. Fujisawa, Y. Nomura, H. Tsuchinaga, Y. Takahashi, Y. Takada, A. Kaga, T. Anai, C. Tsukamoto, H. Seki, T. Muranaka, M. Ishimoto
  • Molecular characterization of cytosolic cysteine synthase in Mimosa pudica; 2017/11
    ANNOUNCEMENT INFO.; J. Plant Res., 131, 319-329
    AUTHOR; Md. Harun-Ur-Rashid, H. Iwasaki, S. Oogai, M. Fukuta, S. Parveen, Md. A. Hossain, T. Anai, H. Oku
  • Chromosomal aneuploidy improves brewing characteristics of sake yeast; 2017/11
    ANNOUNCEMENT INFO.; Applied and Environmental Microbiology, 83, 24, e01620-17
    AUTHOR; M. Kadowaki, Y. Fujimaru, S. Taguchi, J. Ferdouse, K. Sawada, Y. Kimura, Y. Terasawa, G. Agrimi, T. Anai, H. Noguchi, A. Toyoda, A. Fujiyama, T. Akao, H. Kitagaki
  • Confirmation of the pleiotropic control of leaflet shape and number of seeds per pod by the Ln gene in induced soybean mutants; 2017/07
    ANNOUNCEMENT INFO.; Breeding Science, 67, 363-369
    AUTHOR; Takashi Sayama, Takanari Tanabata, Masayasu Saruta, Testsuya Yamada, Toyoaki Anai, Akito Kaga, Masao Ishimoto
  • Identification of quantitative trait loci for owering time by a combination of restriction site–associated DNA sequencing and bulked segregant analysis in soybean; 2017/07
    ANNOUNCEMENT INFO.; Breeding Science, 67, 3, 277-285
    AUTHOR; S. Watanabe, C. Tsukamoto, T. Oshita, T. Yamada, T. Anai, A. Kaga
  • Mutant selection in the self-incompatible plant, radish (Raphanus sativus L. var. sativus), using two-step TILLING.; 2017/07
    ANNOUNCEMENT INFO.; Breeding Science, 67, 3, 268-276
    AUTHOR; Kohzuma K, Chiba M, Nagano S, Anai T, Ueda MU, Oguchi R, Shirai K, Hanada K, Hikosaka K, Fujii N.
  • Genetic and chemical analysis of deep purple flower in soybean; 2017/05
    ANNOUNCEMENT INFO.; Crop science, 57, 4, 1893-1898
    AUTHOR; R. Takahashi*, F. Yan, S. Di, Y. Murai, T. Iwashina, T. Anai
  • Fatty Acid Composition and Distribution in Wild Soybean (Glycine soja) Seeds Collected in Japan.; 2017/05
    ANNOUNCEMENT INFO.; Asian Journal of Plant Sciences, 52-64
    AUTHOR; C.D. Wee, M. Hashiguchi, T. Anai, A. Suzuki and R. Akashii
  • Metabolic switching of astringent and beneficial triterpenoid saponins in soybean is achieved by a loss-of-function mutation in Cytochrome P450 72A69; 2017/02
    ANNOUNCEMENT INFO.; Plant Journal, 89, 527-539
    AUTHOR; R. Yano, K. Takagi, Y. Takada, K. Mukaiyama, C. Tsukamoto, T. Sayama, A. Kaga, T. Anai, S. Sawai, K. Ohyama, K. Saito and M. Ishimoto
  • Metabolite Composition of Grapefruit (Citrus paradisi) Grown in Japan Depends on the Growing Environment and Harvest Period.; 2017/01
    ANNOUNCEMENT INFO.; The Horticulture Journal, 86, 4, 543-551
    AUTHOR; Y. Kimura, M. Naeshiro, Y. Tominaga, T. Anai and F. Komai
  • New Allelic Variant Discovered at Soybean Flower Color Locus W1 Encoding Flavonoid 3′5′-hydroxylase; 2016/06
    ANNOUNCEMENT INFO.; Crop Science, 56, 1506-1513
    AUTHOR; F. Yan, S. Di, Y. Murai, T. Iwashina, T. Anai and R. Takahashi
  • GmCOL1a and GmCOL1b function as flowering repressor in soybean under long-day conditions; 2015/12
    ANNOUNCEMENT INFO.; , 56, 12, 2409-2422
    AUTHOR; D. Cao, Y. Li, S. Lu, J. Wang, H. Nan, X. Li, D. Shi, C. Fang, H. Zhai, X. Yuan, T. Anai, Z. Xia, B. Liu and F. Kong
  • Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing.; 2015/11
    ANNOUNCEMENT INFO.; BMC Genomics, 16, 1014
    AUTHOR; M. Tsuda, A. Kaga, T. Anai, T. Shimizu, T. Sayama, K. Takagi, K. Machita, S. Watanabe, M. Nishimura, N. Yamada, S. Mori, H. Sasaki, H. Kanamori, Y. Katayose, M. Ishimoto
  • Red/far red light controls arbuscular mycorrhizal colonization via jasmonic acid and strigolactone signaling; 2015/11
    ANNOUNCEMENT INFO.; Plant and Cell Physiology, 56, 11, 2100-2109
    AUTHOR; M. Nagata, N. Yamamoto, T. Shigeyama, Y. Terasawa, T. Anai, T. Sakai, S. Inada, S. Arima, A.M. Hirsch , A. Suzuki
  • A novel GmFAD3-2a mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil; 2014/12
    ANNOUNCEMENT INFO.; Breeding Science, 64, 4, 371-377
    AUTHOR; Tomoki Hoshino, Satoshi Watanabe, Yutaka Takagi, Toyoaki Anai
  • Allelic variation of soybean flower color gene W4 encoding dihydroflavonol 4-reductase 2; 2014/03
    ANNOUNCEMENT INFO.; BMC Plant Biology, 14, 58
    AUTHOR; F. Yan, S. Di, F. R. Rodas, T. R. Torrico, Y. Murai, T. Iwashina, T. Anai and R. Takahashi
  • Identification and distribution of Puroindoline b-2 variant gene homologs in Hordeum; 2013/09
    ANNOUNCEMENT INFO.; , 141, 359-368
    AUTHOR; Y. Terasawa, K. Takata, T. Anai and T. M. Ikeda
  • Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes; 2013/02
    ANNOUNCEMENT INFO.; Agronomy, 3, 117-134
    AUTHOR; Y. Tsubokura , H. Matsumura, M. Xu, B. Liu, H. Nakashima, T. Anai, F. Kong, X. Yuan, H. Kanamori, Y. Katayose, R. Takahashi, K. Harada and J. Abe
  • Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1, which regulates photoperiodic flowering; 2012/08
    ANNOUNCEMENT INFO.; Proc. Natl. Acad. Sci. USA, 109, 32, E2155-E2164
    AUTHOR; Zhengjun Xia, Satoshi Watanabe, Tetsuya Yamada, Yasutaka Tsubokura, Hiroko Nakashima, Hong Zhai, Toyoaki Anai, Shusei Sato, Toshimasa Yamazaki, Shixiang Lu, Hongyan Wu, Satoshi Tabata, Kyuya Harada
  • Molecular characterization of two high-palmitic-acid mutant loci induced by X-ray irradiation in soybean; 2012/01
    ANNOUNCEMENT INFO.; Breeding Science, 61, 1, 631-638
    AUTHOR; Toyoaki Anai, Tomoki Hoshino, Naoko Imai and Yutaka Takagi
  • Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean; 2012/01
    ANNOUNCEMENT INFO.; Breeding Science, 61, 1, 462-467
    AUTHOR; Toyoaki Anai
  • The National BioResource Project (NBRP) Lotus and Glycine in Japan; 2012/01
    ANNOUNCEMENT INFO.; Breeding Science, 61, 1, 453-461
    AUTHOR; Masatsugu Hashiguchi, Jun Abe, Toshio Aoki, Toyoaki Anai, Akihiro Suzuki, Ryo Akashi
  • Molecular characterization and marker development of mid-oleic acid mutant M23 for development of high-oleic cultivars of soybean; 2011/10
    ANNOUNCEMENT INFO.; , 130, 544-550
    AUTHOR; Tomoki Hoshino, Nobuhisa Kawashita, Yutaka Takagi, Toyoaki Anai
  • 烏骨鶏と白色レグホーンにおけるプロラクチン遺伝子領域周辺の連鎖不平衡ブロックについて; 2011/10
    ANNOUNCEMENT INFO.; , 48, J2, J63-J68
    AUTHOR; 
  • A map-based cloning strategy employing a residual heterozygous line reveals that the GIGANTEA gene is involved in soybean maturity and flowering.; 2011/06
    ANNOUNCEMENT INFO.; Genetics, 188, 2, 395-407
    AUTHOR; S. Watanabe, Z. Xia, R. Hideshima, Y. Tsubokura, S. Sato, N. Yamanaka, R. Takahashi, T. Anai, S. Tabata, K. Kitamura, K. Harada
  • Novel GmFAD2-1b mutant alleles created by reverse genetics induce drastic elevation of oleic acid content in soybean seeds in combination with GmFAD2-1a mutant alleles; 2010/12
    ANNOUNCEMENT INFO.; Breeding Science, 60, 4, 419-425
    AUTHOR; Tomoki Hoshino, Yutaka Takagi and Toyoaki Anai
  • Phylogenetic relationships of Citrus and its relatives based on rbcL gene sequences; 2010/06
    ANNOUNCEMENT INFO.; Tree Genetics and Genomes, 6, 931-939
    AUTHOR; Tshering Penjor, Toyoaki Anai, Yukio Nagano, Ryoji Matsumoto, Masashi Yamamoto
  • Agrobacterium-mediated transformation of Euphorbia tirucalli callus.; 2010/04
    ANNOUNCEMENT INFO.; Biosci. Biotech. Biochem., 74, 4, 851-853
    AUTHOR; H. Uchida, H. Yamashita, T. Anai, T. Muranaka, K. Ohyama
  • Map-based cloning of the gene associated with soybean maturity locus E3; 2009/08
    ANNOUNCEMENT INFO.; Genetics, 182, 4, 1251-1262
    AUTHOR; Satoshi Watanabe, Rumiko Hideshima, Zhengjun Xia, Yasutaka Tsubokura, Shusei Sato, Yumi Nakamoto, Naoki Yamanaka, Ryoji Takahashi, Masao Ishimoto, Toyoaki Anai, Satoshi Tabata, Kyuya Harada
  • Sequencing and Analysis of Approximately 40,000 Soybean cDNA Clones from a Full-length Enriched cDNA Library; 2008/12
    ANNOUNCEMENT INFO.; DNA Research, 15, 6, 333-346
    AUTHOR; Umezawa, T., Sakurai, T., Totoki, Y., Toyoda, A., Seki, M., Ishiwata, A., Akiyama, K., Kurotani, A., Yoshida, T., Mochida, K., Kasuga, M., Todaka, D., Maruyama, K., Nakashima, K., Enju, A., Mizukado, S., Ahmed, S., Yoshiwara, K., Harada, K., Tsubokura, Y., Hayashi, M., Sato, S., Anai, T., Ishimoto, M., Funatsuki, H., Teraishi, M., Osaki, M., Shinano, T., Akashi, R., Sakaki, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K.
  • Two high-oleic-acid soybean mutants, M23 and KK21, have disrupted microsomal omega-6 fatty acid desaturase, encoded by GmFAD2-1a; 2008/12
    ANNOUNCEMENT INFO.; Breeding Science, 58, 4, 447-452
    AUTHOR; T. Anai, T. Yamada, R. Hideshima, T. Kinoshita, S. M. Rahman, Y. Takagi
  • Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation; 2007/12
    ANNOUNCEMENT INFO.; Plant Cell Reports, 26, 12, 2111-2117
    AUTHOR; Y. Nagano, S. Takao, T. Kudo, E. Iizasa & T. Anai
  • An integrated high-density linkage map of soybean with RFLP, SSR, STS and AFLP markers using a single F2 population; 2007/12
    ANNOUNCEMENT INFO.; DNA Research, 14, 6
    AUTHOR; Z. Xia, Y. Tsubokura, M. Hoshi, M. Hanawa, C. Yano, K. Okamura, T. A. Ahmed, T. Anai, S. Watanabe, T. Kawai, K. G. Hossain, H. Masaki, K. Asai, N. Yamanaka, N. Kubo, K. Kadowaki, S. Sato, S. Tabata, Y. Nagamura, M. Yano, T. Sasaki & K. Harada
  • Hemiphragma heterophyllumの組織培養と二次代謝物生産; 2006
    ANNOUNCEMENT INFO.; , 13, 1
    AUTHOR; 
  • Identification of corresponding genes for three low-a-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed.; 2005
    ANNOUNCEMENT INFO.; Plant Science, 106
    AUTHOR; T. Anai, T. Yamada, T. Kinoshita, S.M. Rahman, & Y. Takagi
  • Analysis of molecular diversity in water chestnut based on RAPD markers.; 2005
    ANNOUNCEMENT INFO.; Biotechnology, 4, 2
    AUTHOR; A. Haque, T. Anai, S. Arima
  • RNA Silencing of the Introduced Coat Protein Gene of Turnip mosaic virus Confers Broad-Spectrum Resistance in Transgenic Arabidopsis.; 2004
    ANNOUNCEMENT INFO.; Phytopathology, 94
    AUTHOR; K. Nomura, K. Ohshima, T. Anai, H. Uekusa & N. Kita
  • Three Novel Soybean Germplasms with Unique Fatty Acid Composition using Multiple Mutant Alleles.; 2004
    ANNOUNCEMENT INFO.; Breeding Science, 54
    AUTHOR; S.M. Rahman, T. Anai, T. Kinoshita, S. Arima & Y. Takagi
  • A Novel Soybean Germplasm with Elevated Saturated Fatty Acids; 2003
    ANNOUNCEMENT INFO.; CROP SCIENCE, 43, 2, 527-531
    AUTHOR; Rahman, S. M / Anai, T / Kinoshita, T / Takagi, Y
  • Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene; 2003
    ANNOUNCEMENT INFO.; PLANT CELL REPORTS, 21, 10, 988-992
    AUTHOR; Anai, T / Koga, M / Tanaka, H / Kinoshita, T / Rahman, S. M / Takagi, Y
  • Combining ability in loci for high oleic and low linolenic acids in soybean; 2001
    ANNOUNCEMENT INFO.; Crop Science, 41, 527-531
    AUTHOR; S.M. Rahman, T. Kinoshita, T. Anai, Y. Takagi
  • Gene transfer into hepatoma cells mediated by galactose-modified alpha-helical peptides; 2000
    ANNOUNCEMENT INFO.; Biomaterials, 21, 1811-1819
    AUTHOR; T. Niidome, M. Urakawa, H. Sato, Y. Takahara, T. Anai, T. Hatakayama, A. Wada, T. Hirayama, H. Aoyagi
  • Development of a reduced linolenate soy mutant by re-irradiation and its genetic analysis; 1999
    ANNOUNCEMENT INFO.; Beeding Science, 49, 1-5
    AUTHOR; Y. Takagi, S.M. Rahman, T. Anai, S.K. Wasala, T. Kinoshita, M. Khalekuzzaman
  • Genetic relationships between loci for palmitate contents in soybean mutants; 1999
    ANNOUNCEMENT INFO.; Journal of Heredity, 90, 423-428
    AUTHOR; S.M. Rahman, T. Kinoshita, T. Anai, Y. Takagi
  • A pair of invertedly repeated genes in Chlamidomonas reinhardtii encodes a zygote-specific protein whose expression is UV-sensitive; 1999
    ANNOUNCEMENT INFO.; Current Genetics, 36, 232-240
    AUTHOR; H. Uchida, L. Suzuki, T. Anai, K. Doi, H. Takano, H. Yamashita, T. Oka, S. Kawano, K. Tomizawa, T. Kawazu, H. Kuroiwa, T. Kuroiwa
  • Classification of sago palm (Metroxylon sagu Rottb.) cultivars by randomly amplified polymorphic DNA analyses; 1999
    ANNOUNCEMENT INFO.; SABRO Journal of Breeding and Genetics, 31, 109-117
    AUTHOR; S. Boonsermsuk, F.S. Jong, T. Anai, K. Hasegawa, S. Hisajima
  • AtGDI2, a novel Arabidopsis gene encoding a Rab GDP dissociation inhibitor; 1998
    ANNOUNCEMENT INFO.; Gene, 206, 137-143
    AUTHOR; T. Ueda, T. Yoshizumi, T. Anai, M. Matsui, H. Uchimiya, A. Nakano
  • Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings; 1998
    ANNOUNCEMENT INFO.; DNA Sequence, 9, 329-333
    AUTHOR; T. Anai, N. Kono, S. Kosemura, S. Yamamura, K. Hasegawa
  • Inter-locus relationship between genes controlling palmitic acid contents in soybean mutants; 1998
    ANNOUNCEMENT INFO.; Breeding Science, 48, 377-381
    AUTHOR; T. Kinoshita, S.M. Rahman, T. Anai, Y. Takagi
  • Establishment of framework P1 clones for map-based cloning and genome sequencing: direct RFLP mapping of large clones; 1998
    ANNOUNCEMENT INFO.; Gene, 225, 31-38
    AUTHOR; D. Shibata, M. Seki, N. Mitsukawa, N. Hayashida, T. Ito, T. Taji, T. Tsuge, M. Matsui, T. Anai, Y.-G. Liu, R.F. Whittier, K. Shinozaki
  • Nucleotide sequence of oat (Avena sativa L.) cDNA encoding an auxin-binding protein (ABP1); 1998
    ANNOUNCEMENT INFO.; DNA Sequence, 8, 235-239
    AUTHOR; T. Anai, N. Kono, R. Takai, T. Tsuge, M. Matsui, S. Kosemura, S. Yamamura, K. Hasegawa
  • The biological roles of small GTPase and interacting proteins in plants; 1998
    ANNOUNCEMENT INFO.; Journal of Plant Research, 111, 257-260
    AUTHOR; H. Uchimiya, T. Anai, T.E. Aspuria, M. Matsui, A. Nakano, T. Ueda
  • Comrarison of abp1 primary sequences from monocotyledonous and dicotyledonous species; 1997
    ANNOUNCEMENT INFO.; Journal of Plant Physiology, 151, 446-449
    AUTHOR; T. Anai, M. Miyata, S. Kosemura, S. Yamamura, T. Tsuge, M. Matsui, H. Uchida, K. Hasegawa
  • Variation in random amplified polymorphic DNA of sago palm (Metroxylon spp.) in Thailand; 1997
    ANNOUNCEMENT INFO.; Japanese Journal of Tropical Agriculture, 41, 89-92
    AUTHOR; S. Boonsermsuk, T. Anai, K. Hasegawa, S. Hisajima
  • Isolation and characterization of an auxin-binding protein gene from radish, and its expression in insect cells; 1997
    ANNOUNCEMENT INFO.; Physiologia Plantarum, 101, 606-611
    AUTHOR; T. Anai, R. Takai, M. Miyata, H. Uchida, S. Kosemura, S. Yamamura, R. Ishizaki, K. Hasegawa
  • Genetic relationships of soybean mutants for different linolenic acid contents; 1997
    ANNOUNCEMENT INFO.; Crop Science, 38, 702-706
    AUTHOR; S.M. Rahman, T. Kinoshita, T. Anai, S. Arima, Y. Takagi
  • Design of photoaffinity reagents for labeling the auxin receptor in maize; 1997
    ANNOUNCEMENT INFO.; Tetrahedron Letter, 38, 2125-2128
    AUTHOR; Kosemura, H. Emori, S. Yamamura, T. Anai, K. Tomita, K. Hasegawa
  • The Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase; 1996
    ANNOUNCEMENT INFO.; , 8, 2079-2091
    AUTHOR; T. Ueda, N. Matsuda, T. Anai, H. Tsukaya, H. Uchimiya, A. Nakano
  • Characterization of the ARA-4 gene: conditional expression under control of promoter of the gene for heat-shock protein HSP81-1 in transgenic Arabidopsis; 1996
    ANNOUNCEMENT INFO.; Molecular and General Genetics, 250, 533-539
    AUTHOR; T. Ueda, T. Anai, H. Tsukaya, A. Hirata, H. Uchimiya
  • Novel DNA prpbes capable of discriminating indica and japonica rice cultivers; 1996
    ANNOUNCEMENT INFO.; DNA Sequence, 6, 303-306
    AUTHOR; S. Oba, M. Hanzawa, T. Anai, M. Umeda, H. Uchimiya
  • Brefeldin A induces the accumulation of unusual membrane structures in elongating pollen tubes of Nicotiana tabacum L.; 1996
    ANNOUNCEMENT INFO.; Journal of Plant Physiology, 149, 683-689
    AUTHOR; T. Ueda, H. Tsukaya, T. Anai, A. Hirata, K. Hasegawa, H. Uchimiya
  • Structure-activity relationship of lepidimoide and its analogues; 1996
    ANNOUNCEMENT INFO.; Phytochemistry, 41, 671-673
    AUTHOR; K. Yamada, T. Anai, S. Kosemura, S. Yamamura, K., Hasegawa
  • A new auxin-inhibiting substance, 4-Cl-6,7-dimethoxy-2-benzoxazolinone, from light-grown maize shoots; 1996
    ANNOUNCEMENT INFO.; Phytochemistory, 42, 273-275
    AUTHOR;  T. Anai, H. Aizawa, N. Ohtake, S. Kosemura, S. Yamamura, K. Hasegawa
  • 植物種子分泌液に検出されたアレロパシー活性における有機物・無機物の貢献度; 1995
    ANNOUNCEMENT INFO.; , 40, 14-19
    AUTHOR; 
  • Lepidimoide, an allelopathic substance in the exudates from germinated seeds; 1995
    ANNOUNCEMENT INFO.; , 39, 1031-1032
    AUTHOR; K. Yamada, T. Anai, K. Hasegawa
  • Sodium chloride stimulatues adenylate kinase level in seedlings of salt-sensitive rice varieties; 1995
    ANNOUNCEMENT INFO.; Journal of Plant Physiology, 147, 277-280
    AUTHOR; P.K. Samarajeewa, M. Kawai, T. Anai, A. Hirai, H. Uchimiya
  • Isolation and characterization of 4-chloro-6,7-dimethoxybenzoxazolin-2-one, a new auxin-inhibiting benzoxazolinone from Zea mays; 1995
    ANNOUNCEMENT INFO.; Chemistry Letter, 1995, 1053-1054
    AUTHOR; Kosemura, H. Emori, S. Yamamura, T. Anai, H. Aizawa, N. Ohtake, K. Hasegawa
  • Immunological analysis of small GTP-binding protein in higher plant cells; 1995
    ANNOUNCEMENT INFO.; Journal of Plant Physiology, 147, 48-52
    AUTHOR; T. Anai, T.E. Aspria, N. Fujii, T. Ueda, M. Matsui, K. Hasegawa, H. Uchimiya
  • Phenotypic instability of transgenic tobacco plants and their progenies expressing Arabidopsis thaliana small GTP-binding protein genes; 1995
    ANNOUNCEMENT INFO.; Molecular and General Genetics, 246, 509-513
    AUTHOR; T.E. Asupuria, T. Anai, N. Fujii, T. Ueda, M. Miyoshi, M. Matsui, H. Uchimiya
  • Chemical studies on 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in connection with 6-methoxy-2-benzoxazolinone, an auxin-inhibiting substance of Zea mays L.; 1994
    ANNOUNCEMENT INFO.; , 35, 8211-8224
    AUTHOR; S. Kosemura, S. Yamamura, T. Anai, K. Hasegawa
  • In vitro mutation analysis of Arabidopsis thaliana small GTP-binding proteins and detection of GAP-like activities in plant cells; 1994
    ANNOUNCEMENT INFO.; FEBS Letter, 346, 175-180
    AUTHOR; T. Anai, M. Matsui, N. Nomura, R. Ishizaki, H. Uchimiya
  • Molecular and structure of ras-related small GTP-binding protein genes of rice plants and GTPase activities of gene products in Escherichia coli; 1993
    ANNOUNCEMENT INFO.; , 332, 282-286
    AUTHOR; S. Kidou, T. Anai, M. Umeda, S. Aotsuka, T. Tsuge, A. Kato, H. Uchimiya
  • Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana; 1991
    ANNOUNCEMENT INFO.; , 105, 159-165
    AUTHOR; T. Hirayama, Y. Imajuku, T. Anai, M. Matsui, A. Oka
  • Isolation and analysis of cDNAs encoding small GTP-binding proteins of Arabidopsis thaliana; 1991
    ANNOUNCEMENT INFO.; Gene, 108, 259-264
    AUTHOR; T. Anai, K. Hasegawa, Y. Watanabe, H. Uchimiya, R. Ishizaki, M. Matsui

Material, Commentary, Editorials, Research Report, A Comprehensive Journal Articles

  • ダイズ突然変異体ライブラリーの増幅を目的としたMultiple Displacement Amplification(MDA)法の最適化; 2010/01
    ANNOUNCEMENT INFO.; , 95, 29-38
    AUTHOR; 
  • The Diversity of Isoflavone contents and In Vitro Antioxidant Activities in Japanese Soybean (Glycine Max (L.) Merr.) Cultivars.; 2010/01
    ANNOUNCEMENT INFO.; Bull. Fac. Agr. Saga Univ., 95, 17-28
    AUTHOR; Pailinrut Chinwangso, Toyoaki Anai, Ryoji Matsumoto
  • Comparison of Regeneration Efficiency of Different Genotypes of Indica Rice Cultivars; 2006/12
    ANNOUNCEMENT INFO.; Bulletin of the Faculty of Agriculture, Saga University, 92
    AUTHOR; A. S. M. T. Abayawickrama & T. Anai
  • Isolation and analysis of storage lipid biosynthesizing genes of soybean.; 2003
    ANNOUNCEMENT INFO.; Proceeding of Japan/Taiwan Symposium on Molecular Biology of Functional Regulation in Plant and Microbe , 70-76
    AUTHOR; T. Anai & Y. Takagi
  • cDNA cloning and molecular phylogenetic analysis of intermediated filament proteins of the flatworm Dugesia japonica. ; 2003
    ANNOUNCEMENT INFO.; Peptide Science , 2002, 377-378
    AUTHOR; T. Mori, R. Gohara, K. Horimoto, T. Ueda, T. Anai, F. Kato, Y. Takasaki & S. Ando
  • ダイズ種子で発現する遺伝子の同定と連鎖分析.; 2003
    ANNOUNCEMENT INFO.; , 6, 18-21
    AUTHOR; 
  • イネ芽生えにおいてジベレリン処理により発現が制御されている遺伝子群の探索; 2002
    ANNOUNCEMENT INFO.; , 87, 95-103
    AUTHOR; 
  • アグロバクテリウムを介したイネ形質転換法の改良; 2000
    ANNOUNCEMENT INFO.; , 85, 155-162
    AUTHOR; 
  • アグロバクテリウムを介した形質転換トマト作出法の改良; 2000
    ANNOUNCEMENT INFO.; , 85, 147-154
    AUTHOR; 
  • Construction of novel fatty acid composition in soybean oil by induced mutation; 1998
    ANNOUNCEMENT INFO.; GAMMA FIELD SYMPOSIA, 37, 17-28
    AUTHOR; Y. Takagi, S.M. Rahman, T. Anai
  • Genetic analysis of restriction fragment length polymorphism on the fatty acid synthesis in soybean mutants and their progenies: I. Low linolenic acid mutants with a microsomal w-3 fatty acid desaturase cDNA as a probe; 1998
    ANNOUNCEMENT INFO.; Bulletin of the Faculty of Agriculture, Saga University, 83, 31-36
    AUTHOR; Y. Yamashita, T. Kinoshita, S.M. Rahman, K. Okada, T. Anai, Y. Takagi
  • Genetic analysis of restriction fragment length polymorphism on the fatty acid synthesis in soybean mutants and their progenies: II. High oleic acid mutants with two microsomal w-6 fatty acid desaturase cDNAs as probes; 1998
    ANNOUNCEMENT INFO.; Bulletin of the Faculty of Agriculture, Saga University, 83, 37-42
    AUTHOR; T. Kinoshita, S.M. Rahman, T. Anai, Y. Takagi
  • Genetic analysis of restriction fragment length polymorphism on the fatty acid synthesis in soybean mutants and their progenies: III. High stearic acid mutants with a stearoyl-acyl carrier protein desaturase cDNA as a probe; 1998
    ANNOUNCEMENT INFO.; Bulletin of the Faculty of Agriculture, Saga University, 83, 43-48
    AUTHOR; S.M. Rahman, T. Kinoshita, T. Anai, Y. Takagi
  • 光屈性研究の新たな展開; 1996
    ANNOUNCEMENT INFO.; , 51, 66-67
    AUTHOR; 
  • Molecular mechanism underlying unstable inheritance of morphological changes in transgenic tobacco plants expressing Arabidopsis small GTP-binding proteins; 1995
    ANNOUNCEMENT INFO.; Proceeding of the US-Japanese joint meeting, 187-197
    AUTHOR; H. Uchimiya, T. Anai, S. Kidou, N. Fujii, T. Ueda, T.E. Aspuria
  • ダイコン下胚軸の重力屈性反応における成長抑制物質ラファヌサニン及びラファヌソールの役割; 1990
    ANNOUNCEMENT INFO.; , 39, 23-29
    AUTHOR; 

General Lectures

  • 大豆品種「トヨシロメ」に見出された高ショ糖遺伝子の特性; 2011/11
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • TILLING法によるダイズGmPhyA1突然変異体の単離とその開花に及ぼす影響; 2011/11
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • TILLING法によるダイズ青色光受容体CRY1突然変異体の単離; 2011/11
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • ダイズ品種「トヨシロメ」に見出された高ショ糖遺伝子の特性; 2011/09
    ANNOUNCEMENT INFO.; , 13, 別2, 215-215
    AUTHOR; 
  • X線照射によって誘発されたダイズ脂肪酸突然変異体におけるDNA変異形態の特徴; 2010/09
    ANNOUNCEMENT INFO.; , 12, 別2, 76-76
    AUTHOR; 
  • ダイズの矮性突然変異遺伝子についての解析; 2010/09
    ANNOUNCEMENT INFO.; , 109, 別2, 77-77
    AUTHOR; 
  • TILLING法を用いたダイズ品種「フクユタカ」からのFT3変異体の単離; 2010/09
    ANNOUNCEMENT INFO.; , 109, 別2, 205-205
    AUTHOR; 
  • MDA法によって増幅を行ったダイズ突然変異体ライブラリーを鋳型としたTILLING法による変異の検出; 2009/12
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • ダイズ種子イソフラボン含量の改変を目指したUDP-グルコース:イソフラボン7-O-グルコシルトランスフェラーゼ突然変異体のスクリーニング; 2009/12
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • ダイズ開花期関連遺伝子座FT2のマップベースクローニング; 2009/09
    ANNOUNCEMENT INFO.; , 11, 2, 84
    AUTHOR; 
  • TILLING法によるダイズ突然変異体ライブラリーからのGmFAD3-2a変異体の単離; 2009/09
    ANNOUNCEMENT INFO.; , 11, 2, 150
    AUTHOR; 
  • TILLING法による超高オレイン酸ダイズ突然変異系統の開発; 2009/09
    ANNOUNCEMENT INFO.; , 11, 2, 167
    AUTHOR; 
  • MDA法によって増幅を行ったダイズ突然変異体ライブラリーを鋳型としたTILLING法による変異の検出; 2009/09
    ANNOUNCEMENT INFO.; , 11, 2, 354
    AUTHOR; 
  • ダイズ遺伝資源中に見出されたFAD2遺伝子ファミリーの多様性; 2009/03
    ANNOUNCEMENT INFO.; , 11, 1, 234
    AUTHOR; 
  • TILLING法を用いたダイズミュータントライブラリーからのFT3突然変異系統の単離; 2008/12
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • MDA法によって増幅したDNAからの変異の検出; 2008/12
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • TILLING法によるダイズ突然変異体ライブラリーのスクリーニング; 2008/10
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • ダイズ開花期関連遺伝子座FT3のマップベースクローニング; 2008/10
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • X-線照射によるダイズ突然変異体ライブラリーの開発; 2007/09
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 組換え型CEL1エンドヌクレアーゼの発現; 2007/09
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 組換え型CEL1エンドヌクレアーゼの発現; 2007
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 「むらゆたか」のX-線照射集団から得られた高ステアリン酸ダイズ突然変異系統; 2007
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • X-線照射によるダイズ突然変異体ライブラリーの開発; 2007
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 日本産在来品種中に見出された高オレイン酸ダイズ遺伝資源の解析; 2007
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • ダイズ脂肪酸生合成突然変異体とその原因遺伝子; 2006
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • ダイズ貯蔵脂質の代謝工学を目指した新たな生物資源の開発; 2006
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 高パルミチン酸ダイズ系統で見出された突然変異遺伝子; 2005
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • RNAi法による高オレイン酸イネの作出; 2005
    ANNOUNCEMENT INFO.; 
    AUTHOR; 

Application of Intellectual Property Rights

  • ダイズ油脂中のオレイン酸含量を増加させる突然変異体及びその原因遺伝子; 2009/06
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 形質転換アイスプラントの作成方法,特許申請; 2005
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • オレリッチ50(大豆品種),品種登録申請; 2005
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 新規なダイズ突然変異体及びそれを用いたダイズ油脂の製造方法,特許申請; 2004
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • オーキシン阻害剤; 1995
    ANNOUNCEMENT INFO.; 
    AUTHOR; 

Review Articles

  • ダイズ突然変異体リソースの整備と新規アリルの開発に関する研究.; 2020/12
    ANNOUNCEMENT INFO.; , 22, 174-177
    AUTHOR; 
  • ダイズ油脂の機能と遺伝的改良.; 2020/11
    ANNOUNCEMENT INFO.; , 4, 18-22
    AUTHOR; 
  • Development and utilization of soybean mutant resources; 2016/11
    ANNOUNCEMENT INFO.; Journal of Crop Research, 61, 67-72
    AUTHOR; Toyoaki Anai
  • 新規の脂肪酸組成を持つダイズ油の突然変異による改良; 2006
    ANNOUNCEMENT INFO.; , 6, 4
    AUTHOR; 
  • 新たな有用物質生産のための植物バイオテクノロジー; 2005
    ANNOUNCEMENT INFO.; , 22, 8
    AUTHOR; 
  • 光屈性研究の新たな展開.; 1996/01
    ANNOUNCEMENT INFO.; , 51, 66-67
    AUTHOR; 

Invited Lecture, Special Lecture

  • ダイズ突然変異体リソースの開発と利用; 2012/09
    ANNOUNCEMENT INFO.; , 83
    AUTHOR; 
  • ダイズ変異体ライブラリーの作出と利用; 2012/09
    ANNOUNCEMENT INFO.; , 14, 2, 17
    AUTHOR; 
  • Soybean mutant resource for reverse genetic study.; 2012/03
    ANNOUNCEMENT INFO.; , 95-95
    AUTHOR; Toyoaki Anai
  • NBRPミヤコグサ・ダイズにおけるダイズリソースの概要について; 2012/02
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • TILLING法に利用できるダイズ突然変異体リソース; 2011/09
    ANNOUNCEMENT INFO.; , 54-54
    AUTHOR; 
  • 新規ダイズ突然変異体リソースとTILLINGによる変異体スクリーニング; 2009/12
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • TILLING法を用いたダイズ(Glycine max)種子脂肪酸組成の代謝工学的改変; 2009/11
    ANNOUNCEMENT INFO.; , 2-3
    AUTHOR; 
  • 突然変異体を活用したダイズ油脂組成の代謝工学的改良; 2009/03
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • 突然変異体を利用したダイズ貯蔵脂質の改良; 2008/12
    ANNOUNCEMENT INFO.; 
    AUTHOR; 
  • Metabolic Engineering of Soybean Seed Oil Quality; 2008/09
    ANNOUNCEMENT INFO.; Asia Pacific Region Environmental Agriculture Symposium
    AUTHOR; Toyoaki Anai

Creation of Technologies and Varieties

  • 大豆 オレリッチ50; 2008/03
    ANNOUNCEMENT INFO.; 
    AUTHOR; Yutaka Takagi, Toyoaki Anai


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