Detailed Information

Research Field/Keywords for Research Field

• Bacteriology

Education

• 1991/03, Graduated
• 1993/03, Master Course, Completed
• 1996/03, Doctor Course, Completed

Employment Experience

• 1996/01 - 1998/12 Postdoctoral Fellowships of Japan Society for the Promotion of Science
• 1999/01 - 1999/03
• 1999/04 - 2000/12
• 2001/01 - 2003/09 Research Associate
• 2003/10 - 2007/03 Research Associate, Faculty of Medicine, Saga University
• 2007/04 -  *  Assistant Professor, Medicine, Faculty of Medicine, Saga University

Field of Specialization

• Bacteriology (including mycology), Biological pharmacy, Molecular biology, Biomedical engineering/ Biomaterial science and engineering

Research Topics and Results

• Leukemia/lymphoma-related factor (LRF) or osteoclast zinc finger protein (OCZF) overexpression promotes osteoclast survival by increasing Bcl-xl mRNA: A novel regulatory mechanism mediated by the RNA binding protein SAM68  2022/09
• Time-dependent efficacy of combination of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus biofilm formation in vitro  2021/03
• 2-Methacryloyloxyethyl Phosphorylcholine Polymer Coating Inhibits Bacterial Adhesion and Biofilm Formation on a Suture: An In Vitro and In Vivo Study 2020/10
• The combination of silver-containing hydroxyapatite coating and vancomycin has a synergistic antibacterial effect on methicillin-resistant Staphylococcus aureus biofilm formation 2020/06
• The combination of silver-containing hydroxyapatite coating and vancomycin has a synergistic antibacterial effect on methicillin-resistant Staphylococcus aureus biofilm formation 2020/02
• Antibacterial Activity of Ag‐Hydroxyapatite Coating Against Hematogenous Infection by Methicillin‐Resistant Staphylococcus aureus in the Rat Femur 2019/12
• Effects of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus in vitro.  2019
• LRF/OCZF regulates survival of osteoclasts via Sam68, a splicing regulator of Bcl-x 2017
• Silver-Containing Hydroxyapatite Coating Reduces Biofilm Formation by Methicillin-Resistant Staphylococcus aureus In Vitro and In Vivo. 2016
• Silver oxide-containing hydroxyapatite coating supports osteoblast function and enhances implant anchorage strength in rat femur. 2015
• Development of a novel matrix-assisted laser desorption/ionization time-of-flight mass spectrum (MALDI-TOF-MS)-based typing method to identify meticillin-resistant Staphylococcus aureus clones 2015
• Comparison of two types of matrix-associated laser desorption/ionization-time of flight mass spectrometers for the identification and typing of Clostridium difficile 2015
• Prevention of bacterial adherence and biofilm formation on a vitamin E-blended, cross-linked polyethylene surface with a poly(2-methacryloyloxyethyl phosphorylcholine) layer 2015
• Establishment of mouse macrophage reporter cell lines which express fluorescent properties under the control of cathepsin K promoter and analysis of osteoclast differentiation 2013
• The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with Legionella pneumophila 2012
• Emergence of optochin resistance among Streptococcus pneumoniae in Japan. 2012
• The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with L. pneumophila. 2012
• Human NAIP restreicts Legionella pneumophila replication accompanied by host cell death 2011/09
• The Transcription Factor FBI-1/OCZF/LRF isExpressed in Osteoclasts and Regulates RANKL-induced Osteoclast Formation In vitro and In vivo. 2011
• Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections. 2010
• Expression of OCZF Directed by the Cathepsin K Promoter Affects Bone Mass and Osteoclast Formation in Transgenic Mice. 2007/09
• The POZ-Zn Transcriptional Regulator OCZF/LRF Is Induced by RANKL and Increases c-Fos Expression in Osteoclastogenesis 2007/09
• Expression and role of POZ-Zn finger transcriptional repressor, OCZF/LRF in osteoclastogenesis. 2006/06

Original Articles

• Leukemia/lymphoma-related factor (LRF) or osteoclast zinc finger protein (OCZF) overexpression promotes osteoclast survival by increasing Bcl-xl mRNA: A novel regulatory mechanism mediated by the RNA binding protein SAM68 ;　2022/09
  ANNOUNCEMENT INFO.;　Lab Invest, 102, 9, 1000-1010
  AUTHOR;　*Xianghe Xu, Takeo Shobuike, Makoto Shiraki, Asana Kamohara, Hirohito Hirata, Masatoshi Murayama, Daisuke Mawatari, Masaya Ueno, Tadatsugu Morimoto, Toshio Kukita, Masaaki Mawatari, Akiko Kukita
  
• Time-dependent efficacy of combination of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus biofilm formation in vitro ;　2021/03
  ANNOUNCEMENT INFO.;　BMC Res Notes, 14, 1, 81
  AUTHOR;　Akira Hashimoto, Hiroshi Miyamoto, Sakumo Kii, Tomoki Kobatake, Takeo Shobuike, Iwao Noda, Motoki Sonohata, Masaaki Mawatari
  
• 2-Methacryloyloxyethyl Phosphorylcholine Polymer Coating Inhibits Bacterial Adhesion and Biofilm Formation on a Suture: An In Vitro and In Vivo Study;　2020/10
  ANNOUNCEMENT INFO.;　, 2020
  AUTHOR;　*Taizo Kaneko, Taku Saito, Takeo Shobuike, Hiroshi Miyamoto, Junpei Matsuda, Kyoko Fukazawa, Kazuhiko Ishihara, Sakae Tanaka , and Toru Moro
  
• The combination of silver-containing hydroxyapatite coating and vancomycin has a synergistic antibacterial effect on methicillin-resistant Staphylococcus aureus biofilm formation;　2020/06
  ANNOUNCEMENT INFO.;　Bone Joint Res, 9, 5, 211-218
  AUTHOR;　Akira Hashimoto, Hiroshi Miyamoto, Tomoki Kobatake, Takema Nakashima, Takeo Shobuike, Masaya Ueno, Takayuki Murakami, Iwao Noda, Motoki Sonohata, Masaaki Mawatari
  
• Antibacterial Activity of Ag‐Hydroxyapatite Coating Against Hematogenous Infection by Methicillin‐Resistant Staphylococcus aureus in the Rat Femur;　2019/12
  ANNOUNCEMENT INFO.;　J Orthop Res. , 37, 12, 2655-2660
  AUTHOR;　Kobatake T , Miyamoto H, Hashimoto A, Ueno M, Nakashima T, Murakami T, Noda I, Shobuike T, Sonohata M, Mawatari M
  
• Silver-Containing Hydroxyapatite Coating Reduces Biofilm Formation by Methicillin-Resistant Staphylococcus aureus In Vitro and In Vivo.;　2016
  ANNOUNCEMENT INFO.;　Biomed Res Int., 2016, Article ID 8070597, 7 pages
  AUTHOR;　○Ueno M, Miyamoto H, Tsukamoto M, Eto S, Noda I, Shobuike T, Kobatake T, Sonohata M, Mawatari M.
  
• Silver oxide-containing hydroxyapatite coating supports osteoblast function and enhances implant anchorage strength in rat femur.;　2015
  ANNOUNCEMENT INFO.;　J Orthop Res., 33, 9, 1391-1397
  AUTHOR;　S Eto, Hiroshi Miyamoto, Takeo Shobuike, Iwao Noda, Takayuki Akiyama, Masatsugu Tsukamoto, Masaya Ueno, Shinsuke Someya, Shunsuke Kawano, Motoki Sonohata, Masaaki Mawatari.
  
• Development of a novel matrix-assisted laser desorption/ionization time-of-flight mass spectrum (MALDI-TOF-MS)-based typing method to identify meticillin-resistant Staphylococcus aureus clones;　2015
  ANNOUNCEMENT INFO.;　J Hosp Infect., 90, 2, p147–155
  AUTHOR;　O. Ueda, S. Tanaka, Z. Nagasawa, H. Hanaki, T. Shobuike, H. Miyamoto
  
• Comparison of two types of matrix-associated laser desorption/ionization-time of flight mass spectrometers for the identification and typing of Clostridium difficile;　2015
  ANNOUNCEMENT INFO.;　Journal of Medical Microbiology, 64, p.1144-1150
  AUTHOR;　Kiyosuke M,Kibe Y, Oho M, Kusaba K, Shimono N, Hota T, Kan DC,Shobuike T, Miyamoto H.
  
• Prevention of bacterial adherence and biofilm formation on a vitamin E-blended, cross-linked polyethylene surface with a poly(2-methacryloyloxyethyl phosphorylcholine) layer;　2015
  ANNOUNCEMENT INFO.;　Acta Biomaterialia, 24, 24-34
  AUTHOR;　*Kyomoto M, Shobuike T, Moro T, Yamane S, Takatori Y, Tanaka S, Miyamoto H, Ishihara K
  
• The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with Legionella pneumophila;　2012
  ANNOUNCEMENT INFO.;　Microbes Infect., 14, 13, 1123-1132
  AUTHOR;　Katagiri N, Shobuike T, Chang B, Kukita A, Miyamoto H.
  
• Emergence of optochin resistance among Streptococcus pneumoniae in Japan.;　2012
  ANNOUNCEMENT INFO.;　Open Journal of Medical Microbiology, 2, 1, 8-15
  AUTHOR;　○Masaki Nagata, Osamu Ueda,Takeo Shobuike,Tetsuro Muratani,Yosuke Aoki, Hiroshi Miyamoto
  
• The Transcription Factor FBI-1/OCZF/LRF isExpressed in Osteoclasts and Regulates RANKL-induced Osteoclast Formation In vitro and In vivo.;　2011
  ANNOUNCEMENT INFO.;　Arthritis Rheum　, 63, 9, 2744-2754
  AUTHOR;　Kukita A, Kukita T, Nagata K, Teramachi J, Li YJ, Yoshida H, Miyamoto H, Gay S, Pessler F and Shobuike T
  
• Development of a novel PCR method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections.;　2010
  ANNOUNCEMENT INFO.;　Oral Surg Oral Med Oral Pathol Oral Radiol Endod., 109, 5, 669-676
  AUTHOR;　Akiyama T, Miyamoto H, Fukuda K, Sano N, Katagiri N, Shobuike T, Kukita A, Yamashita Y, Taniguchi H, Goto M.
  
• Inhibition of Histone Deacetylase Suppresses Osteoclastogenesis and Bone Destruction by Inducing IFN-beta Production;　2005
  ANNOUNCEMENT INFO.;　JOURNAL OF IMMUNOLOGY, 175, 9, 5809-5816
  AUTHOR;　Nakamura, T / Kukita, T / Shobuike, T / Nagata, K / Wu, Z / Ogawa, K / Hotokebuchi, T / Kohashi, O / Kukita, A
  
• Two histone deacetylase inhibitors, trichostatin A and sodium butyrate, suppress differentiation into osteoclasts but not into macrophages;　2003
  ANNOUNCEMENT INFO.;　BLOOD, 101, 9, 3451-3459
  AUTHOR;　Rahman, M. M / Kukita, A / Kukita, T / Shobuike, T / Nakamura, T / Kohashi, O
  

General Lectures

• The combination of silver-containing hydroxyapatite coating and vancomycin has a synergistic antibacterial effect on methicillin-resistant Staphylococcus aureus biofilm formation;　2020/02
  ANNOUNCEMENT INFO.;　ORS 2020 Annual Meeting, 2020. 2.8-11
  AUTHOR;　Hashimoto A , Miyamoto H , Kobatake T , Nakashima T , Shobuike T , Ueno M , Murakami T , Noda I , Sonohata M , Mawatari M
  
• Effects of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus in vitro. ;　2019
  ANNOUNCEMENT INFO.;　ORS 2019 Annual Meeting, 2019. 2.2-5
  AUTHOR;　Hashimoto A, Miyamoto H, Kobatake T, Shobuike T, Ueno M, Murakami T, Noda I, Sonohata M, Mawatari M
  
• LRF/OCZF regulates survival of osteoclasts via Sam68, a splicing regulator of Bcl-x;　2017
  ANNOUNCEMENT INFO.;　ASBMR 2017 Annual Meeting, 2017.9.8‐11
  AUTHOR;　Xu X, Shiraki M., Kamohara A., Shobuike T., Kukita T., Kukita A
  
• Establishment of mouse macrophage reporter cell lines which express fluorescent properties under the control of cathepsin K promoter and analysis of osteoclast differentiation;　2013
  ANNOUNCEMENT INFO.;　Joint meeting of the international Bone and Mineral Research and the Japanese Society for Bone and Mineral Research, May 28-June 1 2013 (Kobe)
  AUTHOR;　Kukita A., Funakubo N,m Shobuike N., Kukita T.
  
• The human apoptosis inhibitor NAIP induces pyroptosis in macrophages infected with L. pneumophila.;　2012
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Katagiri N, Shobuike T, Miyamoto H.
  
• Human NAIP restreicts Legionella pneumophila replication accompanied by host cell death;　2011/09
  ANNOUNCEMENT INFO.;　13th International Union of Microbiological Sciences Congress　2011,9,6-10
  AUTHOR;　Katagiri N, Shobuike T, Miyamoto H
  
• Expression of OCZF Directed by the Cathepsin K Promoter Affects Bone Mass and Osteoclast Formation in Transgenic Mice.;　2007/09
  ANNOUNCEMENT INFO.;　American Society for Bone and Mineral Research 29th Annual Meeting O-099 ホノルル
  AUTHOR;　T. Shobuike, T. Kukita, K. Nagata, J. Teramachi, M. Asagiri, H. Takayanagi, A. Kukita.
  
• The POZ-Zn Transcriptional Regulator OCZF/LRF Is Induced by RANKL and Increases c-Fos Expression in Osteoclastogenesis;　2007/09
  ANNOUNCEMENT INFO.;　American Society for Bone and Mineral Research 29th Annual Meeting W067 ホノルル
  AUTHOR;　A. Kukita, T. Shobuike, M. Asagiri, H. Takayanagi, F. Pessler, K. Matsuo, T. Kukita.
  
• Expression and role of POZ-Zn finger transcriptional repressor, OCZF/LRF in osteoclastogenesis.;　2006/06
  ANNOUNCEMENT INFO.;　第20回国際生化学・分子生物学会議 京都
  AUTHOR;　Akiko Kukita, Takeo Shobuike, Toshio Kukita
  
• A histone deacetylase inhibitor, FR901228, suppresses differatiation osteoclasts but not into macrophages International.Bone and Mineral Sosiety-Japanese Bone and Mineral Society.;　2003/06
  ANNOUNCEMENT INFO.;　2003, 6, 3-7. IBMS-JSBMR 2003Japan day Program and Abstract. JP-22.
  AUTHOR;　Nakamura T,Kukita A,Kukita T, Shobuike T,Kohashi O