Detailed Information

Research Field/Keywords for Research Field

• Molecular spectroscopy, Physical chemistry, Biochemistry

Education

• 2003/03, Graduated
• 2008/03, Doctor Course, Completed

Employment Experience

• 2008/04 - 2010/03 Postdoctoral researcher Chiba University
• 2010/04 - 2012/03 JSPS postdoctoral fellow for research abroad University of California Berkeley
• 2012/04 - 2015/03 Special postdoctoral researcher
• 2015/04 - 2015/04 researcher RIKEN
• 2015/05 - 2016/01 Assistant Professor, Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University
• 2016/02 - 2018/03 Department of Advanced Technology Fusion, Graduate School of Science and Engineering, Saga University
• 2018/04 -  *  Associate Professor, Saga University

Field of Specialization

• Physical chemistry, Biomolecular chemistry

Membership in Academic Societies

• The Chemical Society of Japan, The Spectroscopical Society of Japan

Research Topics and Results

• Molecular Vibrations in Chiral Europium Complexes Revealed by Near-Infrared Raman Optical Activity 2023/11
• Spectroscopic Validation of Crystallographic Structures of a Protein Active Site by Chiroptical Spectroscopy 2023/10
• Raman Optical Activity of Retinal Chromophore in Sensory Rhodopsin II 2023/08
• Heterogeneity of Retinal Chromophore in Chloride Pump Rhodopsins Revealed by Raman Optical Activity 2023/05
• Advanced spectral analysis of complex molecular system 2022/08
• Reisomerization of retinal represents a molecular switch mediating Na+ uptake and release by a bacterial sodium-pumping rhodopsin 2022/08
• 近赤外ラマン分光法の色素タンパク質への応用 2022/08
• ラマン光学活性によるプロテオロドプシン中のレチナール色素の構造解析 2022/07
• Raman Spectroscopy of an Atypical C15-E,syn Bilin Chromophore in Cyanobacteriochrome RcaE 2022/01
• Femtosecond time-resolved absorption study of signaling state of a BLUF protein PixD from the cyanobacterium Synechocystis: Hydrogen-bond rearrangement completes during forward proton-coupled electron transfer 2021/11
• Conformational analysis of a retinal Schiff base chromophore in proteorhodopsin by Raman optical activity 2021/09
• Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE 2021/05
• Prestress in alumina-strengthened porcelain as estimated with Raman scattering spectroscopy 2021/05
• Unno, M., Hirose, Y., Mishima, M., Kikukawa, T., Fujisaewa, T., Tamogami, J. 2021/03
• New probe for porcelain glazes by luminescence at near-infrared excitation 2021/03
• Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport 2020/12
• “Watching” a Molecular Twist in a Protein by Raman Optical Activity 2020/09
• Vibrational optical activity spectroscopy 2020/07
• Raman Optical Activity of Proteins 2020/03
• Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome 2019/10
• Low-temperature Raman spectroscopy of halorhodopsin from Natronomonas pharaonis: Structural discrimination of blue-shifted and red-shifted photoproducts 2019/09
• Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin 2019/07
• Identification of the deprotonated pyrrole nitrogen of the bilin-based photoreceptor by Raman spectroscopy with an advanced computational analysis 2019/03
• Size-dependent separation of graphene oxide by deformation of packed-gel in a chromatographic column 2019/03
• Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin 2018/08
• Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration 2018/08
• Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins 2017/11
• Development of an Azo-Based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy 2017/10
• Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus 2017/04
• Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region 2017/02
• Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates 2016/12
• Raman Optical Activity of Tetra-alanine in the Poly(L-proline) II Type Peptide Conformation 2016/12
• Raman Optical Activity Spectroscopy 2016/10
• Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study 2016/06
• Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-resolved Impulsive Stimulated Raman Spectroscopy 2016/03
• Signaling-state formation mechanism of a BLUF protein PapB from the purple bacterium Rhodopseudomonas palustris studied by femtosecond time-resolved absorption spectroscopy 2014/11
• Time-resolved impulsive Raman study of excited state structures of green fluorescent protein 2014/07
• Structural dynamics of a non-covalent charge transfer complex from femtosecond stimulated Raman spectroscopy 2012/04
• Heavy atom substitution effects in non-aromatic ionic liquids: Ultrafast dynamics and physical properties 2010/07
• Comparison of interionic/intermolecular vibrational dynamics between ionic liquids and concentrated electrolyte solutions 2009/12
• Ultrafast dynamics in aprotic molecular liquids: A femtosecond Raman-induced Kerr effect spectroscopic study 2009/11
• Raman spectroscopic study on solvation of p-aminobenzonitrile in supercritical water and methanol 2009/04
• Solvent effects on the local structure of p-nitroaniline in supercritical water and supercritical alcohols 2008/03
• Raman spectroscopic study of the solvation of decafluorobenzophenone ketyl radical and related compounds in 2-propanol at ambient to supercritical temperatures 2008/02
• Resonance Raman study of the solvation of p-nitroaniline in supercritical water 2006/09
• 超臨界流体中における非線形レーザー分光 2006/06
• Excitation wavelength dependence of the Raman Stokes shift of N,N-dimethyl-p-nitroaniline 2006/05
• Raman spectroscopic study on solvation of diphenylcyclopropenone and phenol blue in room temperature ionic liquids 2006/04
• Development and application of the multipurpose optical flow cell under supercritical condition of water 2005/09
• Acceptor number of room temperature ionic liquid determined by the Raman spectrum of diphenylcyclopropenone 2005/02

Books

• Advanced spectral analysis of complex molecular system;　2022/08
  ANNOUNCEMENT INFO.;　In Molecular and Laser Spectroscopy: Advances and Applications: Volume 3; Gupta, V. P. Ed.; Elsevier Inc.; pp 25-54 (2022)
  AUTHOR;　T. Fujisawa, M. Unno
  
• Vibrational optical activity spectroscopy;　2020/07
  ANNOUNCEMENT INFO.;　In Molecular and Laser Spectroscopy: Advances and Applications: Volume 2; Gupta, V. P. & Ozaki, Y. Eds.; Elsevier Inc.; pp 41-82 (2020)
  AUTHOR;　V.P. Gupta et al.
  

Original Articles

• Molecular Vibrations in Chiral Europium Complexes Revealed by Near-Infrared Raman Optical Activity;　2023/11
  ANNOUNCEMENT INFO.;　Adv. Sci. 2305521 (2023)
  AUTHOR;　Wu, T., Bouř, P., Fujisawa, T., Unno, M.
  
• Spectroscopic Validation of Crystallographic Structures of a Protein Active Site by Chiroptical Spectroscopy;　2023/10
  ANNOUNCEMENT INFO.;　J. Phys. Chem. Lett. 14, 9304-9309 (2023)
  AUTHOR;　Fujisawa, T., Shingae, T., Ren, J., Haraguchi, S., Hanamoto, T., Hoff, W. D., Unno, M.
  
• Raman Optical Activity of Retinal Chromophore in Sensory Rhodopsin II;　2023/08
  ANNOUNCEMENT INFO.;　J. Phys. Chem. B 127, 7244-7250 (2023)
  AUTHOR;　Nishikawa, K., Kuroiwa, R., Tamogami, J., Unno, M., Fujisawa, T.
  
• Heterogeneity of Retinal Chromophore in Chloride Pump Rhodopsins Revealed by Raman Optical Activity;　2023/05
  ANNOUNCEMENT INFO.;　J. Phys. Chem. B 127, 4775-4782 (2023)
  AUTHOR;　Ohya, M., Kikukawa, T., Matsuo, J., Tsukamoto, T., Nagaura, R., Fujisawa, T., Unno, M.
  
• Reisomerization of retinal represents a molecular switch mediating Na+ uptake and release by a bacterial sodium-pumping rhodopsin;　2022/08
  ANNOUNCEMENT INFO.;　J. Biol. Chem. 296, 100792 (2022)
  AUTHOR;　T. Fujisawa, K. Kinoue, R. Seike, T. Kikukawa, M. Unno
  
• Raman Spectroscopy of an Atypical C15-E,syn Bilin Chromophore in Cyanobacteriochrome RcaE;　2022/01
  ANNOUNCEMENT INFO.;　J. Phys. Chem. B 126, 813-821 (2022)
  AUTHOR;　Okuda, Y., Miyoshi, R., Kamo, T., Fujisawa, T., Nagae, T., Mishima, M., Eki, T., Hirose, Y., Unno, M.
  
• Femtosecond time-resolved absorption study of signaling state of a BLUF protein PixD from the cyanobacterium Synechocystis: Hydrogen-bond rearrangement completes during forward proton-coupled electron transfer;　2021/11
  ANNOUNCEMENT INFO.;　J. Phys. Chem. B 125, 12154-12165 (2021)
  AUTHOR;　Fujisawa, T., Masuda, S., Takeuchi, S., Tahara, T.
  
• Conformational analysis of a retinal Schiff base chromophore in proteorhodopsin by Raman optical activity;　2021/09
  ANNOUNCEMENT INFO.;　J. Phys. Chem. Lett. 12, 9564-9568 (2021)
  AUTHOR;　
  
• Structural basis of the protochromic green/red photocycle of the chromatic acclimation sensor RcaE;　2021/05
  ANNOUNCEMENT INFO.;　Proc. Natl. Acad. Sci. USA 118, e2024583118 (2021)
  AUTHOR;　Nagae, T., Unno, M., Koizumi, T., Miyanoiri, Y., Fujisawa, T., Masui, K., Kamo, T., Wada, K., Eki, T., Ito, Y., Hirose, Y., Mishima, M.
  
• Prestress in alumina-strengthened porcelain as estimated with Raman scattering spectroscopy;　2021/05
  ANNOUNCEMENT INFO.;　J. Ceram. Soc. Jpn. 129, 267-270 (2021)
  AUTHOR;　Akatsu, T., Fujisawa, T., Unno, M.
  
• New probe for porcelain glazes by luminescence at near-infrared excitation;　2021/03
  ANNOUNCEMENT INFO.;　ACS Omega 6, 7829-7833 (2021)
  AUTHOR;　Kamura, S., Tani, T., Matsuo, H., Onaka, T., Fujisawa, T., Unno, M.
  
• Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport;　2020/12
  ANNOUNCEMENT INFO.;　Phys. Chem. Chem. Phys. 23, 2072-2079 (2021)
  AUTHOR;　Y. Nakamizo, T. Fujisawa, T. Kikukawa, A. Okamura, H. Baba, M. Unno
  
• “Watching” a Molecular Twist in a Protein by Raman Optical Activity;　2020/09
  ANNOUNCEMENT INFO.;　J. Phys. Chem. Lett. 11, 8579-8584 (2020)
  AUTHOR;　J. Matsuo, T. Kikukawa, T. Fujisawa, W. D. Hoff, M. Unno
  
• Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome;　2019/10
  ANNOUNCEMENT INFO.;　J Biol Chem. 2019, 294, 18909-18922.
  AUTHOR;　T. Sato, T. Kikukawa, R. Miyoshi, K. Kajimoto, C. Yonekawa, T. Fujisawa, M. Unno, T. Eki, Y. Hirose
  
• Low-temperature Raman spectroscopy of halorhodopsin from Natronomonas pharaonis: Structural discrimination of blue-shifted and red-shifted photoproducts;　2019/09
  ANNOUNCEMENT INFO.;　Biochemistry 58, 4159-4167, 2019
  AUTHOR;　T. Fujisawa, H. Kiyota, T. Kikukawa, M. Unno
  
• Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin;　2019/07
  ANNOUNCEMENT INFO.;　Scientific Reports 9, 10711, 2019
  AUTHOR;　A. Iizuka, K. Kajimoto, T. Fujisawa, T. Tsukamoto, T. Aizawa, N. Kamo, K.-H. Jung, M. Unno, M. Demura, T. Kikukawa*
  
• Identification of the deprotonated pyrrole nitrogen of the bilin-based photoreceptor by Raman spectroscopy with an advanced computational analysis;　2019/03
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry B, 123, 3242-3247, 2019
  AUTHOR;　S. Osoegawa, R. Miyoshi, K. Watanabe, Y. Hirose, T. Fujisawa, M. Ikeuchi,M. Unno
  
• Size-dependent separation of graphene oxide by deformation of packed-gel in a chromatographic column;　2019/03
  ANNOUNCEMENT INFO.;　
  AUTHOR;　Y. Takaoka, S. Esaki, K. Sakaguchi, T. Fujisawa, M. Unno, S. Morisada, K. Ohto, H. Kawakita
  
• Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin;　2018/08
  ANNOUNCEMENT INFO.;　FEBS Letters 592(18) 3054-3061
  AUTHOR;　T. Fujisawa , M. Abe, J. Tamogami , T. Kikukawa, N. Kamo and M. Unno
  
• Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration;　2018/08
  ANNOUNCEMENT INFO.;　Proc. Natl. Acad. Sci. USA 115(35) 8671-8675
  AUTHOR;　Haraguchi, S., Shingae, T., Fujisawa, T., Kasai, N., Kumauchi, M., Hanamoto, T., Hoff, W. D., Unno, M.
  
• Development of an Azo-Based Photosensitizer Activated under Mild Hypoxia for Photodynamic Therapy;　2017/10
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 139, 13713-13719, 2017.
  AUTHOR;　W. Piao, K. Hanaoka, T. Fujisawa, S. Takeuchi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, T. Nagano, Y. Urano
  
• Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus;　2017/04
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry B, 121, 4431-4437, 2017.
  AUTHOR;　K. Kajimoto, T. Kikukawa, H. Nakashima, H. Yamaryo, Y. Saito, T. Fujisawa, M. Demura, M. Unno,
  
• Broadband Stimulated Raman Spectroscopy in the Deep Ultraviolet Region;　2017/02
  ANNOUNCEMENT INFO.;　Chemical Physics Letters, , 683, 543-546, 2017
  AUTHOR;　 H. Kuramochi, T. Fujisawa, S. Takeuchi, T. Tahara
  
• Development of an Azoreductase-based Reporter System with Synthetic Fluorogenic Substrates;　2016/12
  ANNOUNCEMENT INFO.;　ACS Chemical Biology, 12, 558-563, 2017.
  AUTHOR;　N. Shin, K. Hanaoka, W. Piao, T. Miyakawa, T. Fujisawa, S. Takeuchi, S. Takahashi, T. Komatsu, T. Ueno, T. Terai, T. Tahara, M. Tanokura, T. Nagano, Y. Urano
  
• Raman Optical Activity of Tetra-alanine in the Poly(L-proline) II Type Peptide Conformation;　2016/12
  ANNOUNCEMENT INFO.;　 Physical Chemistry Chemical Physics, 19, 2078-2086 (2016).
  AUTHOR;　 M. Furuta, T. Fujisawa, H. Urago, T. Eguchi, T. Shingae, S. Takahashi, E. W. Blanch, M. Unno
  
• Ultrafast Dynamics in Aromatic Cation Based Ionic Liquids: A Femtosecond Raman-Induced Kerr Effect Spectroscopic Study;　2016/06
  ANNOUNCEMENT INFO.;　Bulletin of the Chemical Society of Japan 2016, 89, 1106–1128 (2016)
  AUTHOR;　Hideaki Shirota, Shohei Kakinuma, Kotaro Takahashi, Akito Tago, Hocheon Jeong, and Tomotsumi Fujisawa
  
• Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-resolved Impulsive Stimulated Raman Spectroscopy;　2016/03
  ANNOUNCEMENT INFO.;　Journal of the American Chemical Society, 138, 3942–3945 (2016)
  AUTHOR;　T. Fujisawa, H. Kuramochi, H. Hosoi, S. Takeuchi, T. Tahara
  
• Signaling-state formation mechanism of a BLUF protein PapB from the purple bacterium Rhodopseudomonas palustris studied by femtosecond time-resolved absorption spectroscopy;　2014/11
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry B, 118, 14761−14773 (2014)
  AUTHOR;　T. Fujisawa, S. Takeuchi, S. Masuda, T. Tahara
  
• Structural dynamics of a non-covalent charge transfer complex from femtosecond stimulated Raman spectroscopy;　2012/04
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry B, 116, 10453–10460, (2012).
  AUTHOR;　T. Fujisawa, M. Creelman, R. A. Mathies
  
• Heavy atom substitution effects in non-aromatic ionic liquids: Ultrafast dynamics and physical properties;　2010/07
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry B, 114, 9400-9412 (2010)
  AUTHOR;　H. Shirota, H. Fukazawa, T. Fujisawa, J. F. Wishart
  
• Comparison of interionic/intermolecular vibrational dynamics between ionic liquids and concentrated electrolyte solutions;　2009/12
  ANNOUNCEMENT INFO.;　Journal of Chemical Physics, 131, 244519 (2009)
  AUTHOR;　T. Fujisawa, K. Nishikawa, H. Shirota
  
• Ultrafast dynamics in aprotic molecular liquids: A femtosecond Raman-induced Kerr effect spectroscopic study;　2009/11
  ANNOUNCEMENT INFO.;　Bulletin of the Chemical Society of Japan, 82, 1347-1366 (2009)
  AUTHOR;　H. Shirota, T. Fujisawa, H. Fukazawa, K. Nishikawa
  
• Raman spectroscopic study on solvation of p-aminobenzonitrile in supercritical water and methanol;　2009/04
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry A, 113, 3143-3154, (2009)
  AUTHOR;　K. Osawa, T. Hamamoto, T. Fujisawa, M. Terazima, H. Sato, Y. Kimura
  
• Solvent effects on the local structure of p-nitroaniline in supercritical water and supercritical alcohols;　2008/03
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry A, 112, 5515-5526 (2008)
  AUTHOR;　T. Fujisawa, M. Terazima, Y. Kimura
  
• Raman spectroscopic study of the solvation of decafluorobenzophenone ketyl radical and related compounds in 2-propanol at ambient to supercritical temperatures;　2008/02
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry A, 112, 1914-1921, (2008)
  AUTHOR;　T. Fujisawa, T. Ito, M. Terazima, Y. Kimura
  
• Resonance Raman study of the solvation of p-nitroaniline in supercritical water;　2006/09
  ANNOUNCEMENT INFO.;　Chemical Physics Letters, 430, 303-308, (2006).
  AUTHOR;　T. Fujisawa, M. Terazima, Y. Kimura, M. Maroncelli
  
• Excitation wavelength dependence of the Raman Stokes shift of N,N-dimethyl-p-nitroaniline;　2006/05
  ANNOUNCEMENT INFO.;　Journal of Chemical Physics, 124, 184503 (2006)
  AUTHOR;　T. Fujisawa, M. Terazima, Y. Kimura
  
• Raman spectroscopic study on solvation of diphenylcyclopropenone and phenol blue in room temperature ionic liquids;　2006/04
  ANNOUNCEMENT INFO.;　Journal of Physical Chemistry A, 110, 6164-6172, (2006)
  AUTHOR;　T. Fujisawa, M. Fukuda, M. Terazima, Y. Kimura
  
• Acceptor number of room temperature ionic liquid determined by the Raman spectrum of diphenylcyclopropenone;　2005/02
  ANNOUNCEMENT INFO.;　Chemistry Letters, 34, 338-339, (2005)
  AUTHOR;　Y. Kimura, M. Fukuda, T. Fujisawa, M. Terazima
  

Material, Commentary, Editorials, Research Report, A Comprehensive Journal Articles

• 近赤外ラマン分光法の色素タンパク質への応用;　2022/08
  ANNOUNCEMENT INFO.;　
  AUTHOR;　藤澤知績、海野雅司
  
• ラマン光学活性によるプロテオロドプシン中のレチナール色素の構造解析;　2022/07
  ANNOUNCEMENT INFO.;　藤澤知績、 海野雅司
  AUTHOR;　
  
• Unno, M., Hirose, Y., Mishima, M., Kikukawa, T., Fujisaewa, T., Tamogami, J.;　2021/03
  ANNOUNCEMENT INFO.;　
  AUTHOR;　
  
• Time-resolved impulsive Raman study of excited state structures of green fluorescent protein;　2014/07
  ANNOUNCEMENT INFO.;　Ultrafast Phenomena XIX, Proceedings of the 19th International Conference, 539-542 (2014)
  AUTHOR;　T. Fujisawa, H. Kuramochi, S. Takeuchi, T. Tahara
  
• 超臨界流体中における非線形レーザー分光;　2006/06
  ANNOUNCEMENT INFO.;　高圧力の科学と技術，日本高圧力学会，16巻2号，87-94，(2006).
  AUTHOR;　木村佳文，網田　富士嗣，藤澤知績
  
• Development and application of the multipurpose optical flow cell under supercritical condition of water;　2005/09
  ANNOUNCEMENT INFO.;　Proceedings of the 14th International Conference on the Properties of Water and Steam, 445-450 (2005)
  AUTHOR;　T. Fujisawa, E. Maru , F. Amita, M. Harada, T. Uruga, and Y. Kimura
  

Review Articles

• Raman Optical Activity of Proteins;　2020/03
  ANNOUNCEMENT INFO.;　Encyclopedia of Analytical Chemistry: Applications, Theory and Instrumentation
  AUTHOR;　Tomotsumi Fujisawa
  
• Light-induced chromophore and protein responses and mechanical signal transduction of BLUF proteins;　2017/11
  ANNOUNCEMENT INFO.;　Biophysical Reviews, 1-11, 2017.
  AUTHOR;　T. Fujisawa, S. Masuda
  
• Raman Optical Activity Spectroscopy;　2016/10
  ANNOUNCEMENT INFO.;　分光研究、第65巻、第4号、218-231
  AUTHOR;　Tomotsumi Fujisawa, Masashi Unno