日本語フィールド
著者：*Tomita R, Hayama T, Nishijo N, Sogawa R, Shimanoe C, Fujioka T題名：Liquid Chromatographic Determination of o-Phosphoethanolamine in Human Plasma Using Fluorescent Derivatization発表情報：Chem Pharm Bull (Tokyo) 巻: 71 号: 1 ページ: 10 - 14キーワード：fluorescence derivatization; human plasma; hydrophilic interaction liquid chromatography (HILIC); o-phosphoethanolamine概要：In this study, an HPLC analysis method using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) was developed for the determination of o-phosphoethanolamine (PEA), which is a potential biomarker for the diagnosis of major depressive disorder, in human plasma sample. After PEA was derivatized with AQC under mild conditions, the obtained derivative was subjected to purification with a titanium dioxide-modified monolithic silica spin column (MonoSpin® TiO). The eluate from the MonoSpin® TiO was directly injected into an amide-type hydrophilic interaction liquid chromatography (HILIC) column-equipped HPLC system, and the resulting derivative could be separated on the column under alkaline mobile phase conditions and subsequently detected fluorometrically at excitation and emission wavelengths of 250 and 395nm, respectively. The limit of detection and limit of quantification for a 10µL injection volume of PEA were 0.052 and 0.17µM, respectively. The method was validated at 0.2, 1.0, and 5.0nmol/mL levels in plasma sample, and the precision values were 2.0–6.6% as relative standard deviation and the correlation coefficient (r) of the calibration curve was 0.9995. Furthermore, applicability of this method was demonstrated by analyzing PEA levels in plasma samples from mental illness patients.抄録：英語フィールド
Author：*Tomita R, Hayama T, Nishijo N, Sogawa R, Shimanoe C, Fujioka TTitle：Liquid Chromatographic Determination of o-Phosphoethanolamine in Human Plasma Using Fluorescent DerivatizationAnnouncement information:Chem Pharm Bull (Tokyo) Vol: 71 Issue: 1 Page: 10 - 14Keyword：fluorescence derivatization; human plasma; hydrophilic interaction liquid chromatography (HILIC); o-phosphoethanolamineAn abstract：In this study, an HPLC analysis method using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) was developed for the determination of o-phosphoethanolamine (PEA), which is a potential biomarker for the diagnosis of major depressive disorder, in human plasma sample. After PEA was derivatized with AQC under mild conditions, the obtained derivative was subjected to purification with a titanium dioxide-modified monolithic silica spin column (MonoSpin® TiO). The eluate from the MonoSpin® TiO was directly injected into an amide-type hydrophilic interaction liquid chromatography (HILIC) column-equipped HPLC system, and the resulting derivative could be separated on the column under alkaline mobile phase conditions and subsequently detected fluorometrically at excitation and emission wavelengths of 250 and 395nm, respectively. The limit of detection and limit of quantification for a 10µL injection volume of PEA were 0.052 and 0.17µM, respectively. The method was validated at 0.2, 1.0, and 5.0nmol/mL levels in plasma sample, and the precision values were 2.0–6.6% as relative standard deviation and the correlation coefficient (r) of the calibration curve was 0.9995. Furthermore, applicability of this method was demonstrated by analyzing PEA levels in plasma samples from mental illness patients.