日本語フィールド
著者：*Kenichi Arai, Daiki Murata, Shoko Takao, Koichi Nakayama題名：Fabrication of Cardiac Constructs Using Bio-3D Printer 発表情報：Methods Mol Biol 巻: 2320 ページ: 53-63キーワード：Bio-3D printer; Biofabrication; Cardiac construct; Human induced pluripotent stem cell-derived cardiomyocytes; Scaffold-free概要：The fabrication of three-dimensional (3D) cardiac tissue using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is useful not only for regenerative medicine, but also for drug discovery. Here, we report a bio-3D printer that can fabricate tubular cardiac constructs using only human iPSC-CMs. Protocols to evaluate the contractile force and response to electrical stimulation in the cardiac constructs are described. We confirmed that the constructs can be applied for transplantation or drug response testing. In the near future, we expect that the constructs will be used as alternatives for heart transplantation and in animal experiments for new drug development. 抄録：英語フィールド
Author：*Kenichi Arai, Daiki Murata, Shoko Takao, Koichi NakayamaTitle：Fabrication of Cardiac Constructs Using Bio-3D Printer Announcement information:Methods Mol Biol Vol: 2320 Page: 53-63Keyword：Bio-3D printer; Biofabrication; Cardiac construct; Human induced pluripotent stem cell-derived cardiomyocytes; Scaffold-freeAn abstract：The fabrication of three-dimensional (3D) cardiac tissue using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is useful not only for regenerative medicine, but also for drug discovery. Here, we report a bio-3D printer that can fabricate tubular cardiac constructs using only human iPSC-CMs. Protocols to evaluate the contractile force and response to electrical stimulation in the cardiac constructs are described. We confirmed that the constructs can be applied for transplantation or drug response testing. In the near future, we expect that the constructs will be used as alternatives for heart transplantation and in animal experiments for new drug development. 