日本語フィールド
著者：*Kunihito Arai, Masahiro Sakaguchi, Shunsuke Yui, Tomoaki Kitano, Miho Miyata, Mayumi Yogosawa, Kazutaka Nakayama, Kenji Tajika, Kensuke Usuki, Junya Kuroda, Nobuhiko Uoshima, Yutaka Kobayashi, Hitoji Uchiyama, Yasushi Kubota, Shinya Kimura, Shinichiro Mori, Mitsuharu Hirai, Satoshi Wakita, Hiroki Yamaguchi題名：Simultaneous detection of JAK2, CALR, and MPL mutations and quantitation of JAK2 V617F allele burden in myeloproliferative neoplasms using the quenching probe-Tm method in i-densy IS-5320発表情報：Int J Lab Hematol 巻: 44 号: 6 ページ: 1102-1110キーワード：CALR; JAK2; MPL; gene testing; i-densy IS-5320; myeloproliferative neoplasms概要：Introduction: Accurate detection of myeloproliferative neoplasms (MPN)-associated gene mutations is necessary to correctly diagnose MPN. However, conventional gene testing has various limitations, including the requirement of skilled technicians, cumbersome experimental procedures, and turnaround time of several days. The gene analyzer i-densy IS-5320 allows gene testing using the quenching probe-Tm method. Specifically, pretreatment of samples including DNA extraction, amplification and detection of genes, and analysis of results are performed in a fully automatic manner after samples and test reagents are added into this system, which is compact and can be easily installed in a laboratory. The aim of this study is to investigate the sensitivity and specificity associated with the simultaneous detection of MPN-associated gene mutations.
Methods: We conducted an analysis of MPN-associated genes using i-densy IS-5320. We analyzed 384 samples (171 JAK2 V617F mutations, 10 JAK2 exon12 mutations, 104 CALR mutations, and 26 MPL mutations) that had been examined using conventional approaches such as allele-specific polymerase chain reaction (PCR), droplet digital PCR, and the direct sequencing method.
Results: The detection accuracy of JAK2 V617F, JAK2 exon 12, CALR, and MPL was 100.0% (383/383), 99.7% (383/384), 100.0% (370/370), and 99.7% (377/378), respectively. There was a strong positive correlation between the JAK2 V617F allele burden measured using conventional methods and i-densy IS-5320 (r = .989).
Conclusion: Overall, i-densy IS-5320 exhibited good accuracy in terms of analyzing MPN-associated genes; thus, it can serve as a replacement for conventional methods of MPN-associated gene testing.抄録：英語フィールド
Author：*Kunihito Arai, Masahiro Sakaguchi, Shunsuke Yui, Tomoaki Kitano, Miho Miyata, Mayumi Yogosawa, Kazutaka Nakayama, Kenji Tajika, Kensuke Usuki, Junya Kuroda, Nobuhiko Uoshima, Yutaka Kobayashi, Hitoji Uchiyama, Yasushi Kubota, Shinya Kimura, Shinichiro Mori, Mitsuharu Hirai, Satoshi Wakita, Hiroki YamaguchiTitle：Simultaneous detection of JAK2, CALR, and MPL mutations and quantitation of JAK2 V617F allele burden in myeloproliferative neoplasms using the quenching probe-Tm method in i-densy IS-5320Announcement information:Int J Lab Hematol Vol: 44 Issue: 6 Page: 1102-1110Keyword：CALR; JAK2; MPL; gene testing; i-densy IS-5320; myeloproliferative neoplasmsAn abstract：Introduction: Accurate detection of myeloproliferative neoplasms (MPN)-associated gene mutations is necessary to correctly diagnose MPN. However, conventional gene testing has various limitations, including the requirement of skilled technicians, cumbersome experimental procedures, and turnaround time of several days. The gene analyzer i-densy IS-5320 allows gene testing using the quenching probe-Tm method. Specifically, pretreatment of samples including DNA extraction, amplification and detection of genes, and analysis of results are performed in a fully automatic manner after samples and test reagents are added into this system, which is compact and can be easily installed in a laboratory. The aim of this study is to investigate the sensitivity and specificity associated with the simultaneous detection of MPN-associated gene mutations.
Methods: We conducted an analysis of MPN-associated genes using i-densy IS-5320. We analyzed 384 samples (171 JAK2 V617F mutations, 10 JAK2 exon12 mutations, 104 CALR mutations, and 26 MPL mutations) that had been examined using conventional approaches such as allele-specific polymerase chain reaction (PCR), droplet digital PCR, and the direct sequencing method.
Results: The detection accuracy of JAK2 V617F, JAK2 exon 12, CALR, and MPL was 100.0% (383/383), 99.7% (383/384), 100.0% (370/370), and 99.7% (377/378), respectively. There was a strong positive correlation between the JAK2 V617F allele burden measured using conventional methods and i-densy IS-5320 (r = .989).
Conclusion: Overall, i-densy IS-5320 exhibited good accuracy in terms of analyzing MPN-associated genes; thus, it can serve as a replacement for conventional methods of MPN-associated gene testing.