日本語フィールド
著者：*Tomogane M, Omura M, Sano Y, Shimizu D, Toda Y, Hosogi S, Kimura S, Ashihara E題名：Expression level of BTN3A1 on the surface of CD14 + monocytes is a potential predictor of γδ T cell expansion efficiency 発表情報：Biochem Biophys Res Commun 巻: 588 ページ: 47-54キーワード：BTN3A1; Monocytes; Vγ9Vδ2 T cells; γδ T cells概要：Human γδ T cells expressing Vγ9Vδ2 T cell receptors exert a robust response to pathogens and malignant cells. These cells are activated by BTN3A1, which is expressed by pathogen-derived phosphoantigens (pAgs) or host-derived pAgs that accumulate in transformed cells or in cells exposed to aminobisphosphonates. Activated Vδ2 (+) T cells exert multiple effector functions; therefore, they are a promising candidate for immunotherapy. However, not all donors have γδ T cells with adequate proliferative activity. Here, we performed ex vivo culture of γδ T cells from 20 healthy donors and explored factors that may affect their expansion efficiency. Consistent with previous studies, we found that amplification of γδ T cells requires CD14+ monocytes to act as accessory cells. We also show here that surface expression of BTN3A1 by monocytes correlates positively with γδ T cell expansion. Moreover, treatment with BTN3A1-Fc increased the expansion efficiency of peripheral blood mononuclear cells (PBMCs) from donors harboring γδ T cells with poor expansion capacity. Taken together, the data suggest that the level of BTN3A1 expressed on the surface of monocytes is a useful biomarker for predicting the degree of expansion of γδ T cells. 抄録：英語フィールド
Author：*Tomogane M, Omura M, Sano Y, Shimizu D, Toda Y, Hosogi S, Kimura S, Ashihara ETitle：Expression level of BTN3A1 on the surface of CD14 + monocytes is a potential predictor of γδ T cell expansion efficiency Announcement information:Biochem Biophys Res Commun Vol: 588 Page: 47-54Keyword：BTN3A1; Monocytes; Vγ9Vδ2 T cells; γδ T cellsAn abstract：Human γδ T cells expressing Vγ9Vδ2 T cell receptors exert a robust response to pathogens and malignant cells. These cells are activated by BTN3A1, which is expressed by pathogen-derived phosphoantigens (pAgs) or host-derived pAgs that accumulate in transformed cells or in cells exposed to aminobisphosphonates. Activated Vδ2 (+) T cells exert multiple effector functions; therefore, they are a promising candidate for immunotherapy. However, not all donors have γδ T cells with adequate proliferative activity. Here, we performed ex vivo culture of γδ T cells from 20 healthy donors and explored factors that may affect their expansion efficiency. Consistent with previous studies, we found that amplification of γδ T cells requires CD14+ monocytes to act as accessory cells. We also show here that surface expression of BTN3A1 by monocytes correlates positively with γδ T cell expansion. Moreover, treatment with BTN3A1-Fc increased the expansion efficiency of peripheral blood mononuclear cells (PBMCs) from donors harboring γδ T cells with poor expansion capacity. Taken together, the data suggest that the level of BTN3A1 expressed on the surface of monocytes is a useful biomarker for predicting the degree of expansion of γδ T cells. 