日本語フィールド
著者：Hiroo Katsuya, Lucy B M Cook, Aileen G Rowan, Anat Melamed, Jocelyn Turpin, Jumpei Ito, Saiful Islam, Paola Miyazato, Benjy Jek Yang Tan, Misaki Matsuo, Toshikazu Miyakawa, Hirotomo Nakata, Shuzo Matsushita, Graham P Taylor, Charles R M Bangham, Shinya Kimura, Yorifumi Satou題名：Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals 発表情報：J Infect Dis 巻: 225 号: 2 ページ: 317-326キーワード：HIV-1; HTLV-1; High-throughput sequencing; Integration site analysis; coinfection概要：Background: Coinfection with HIV-1 and HTLV-1 diminishes the value of the CD4 + T-cell count in diagnosing AIDS, and increases the rate of HTLV-1-associated myelopathy. It remains elusive how HIV-1/HTLV-1 coinfection is related to such clinical characteristics. Here, we investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites (ISs) and the clonal expansion.
Methods: We extracted DNA from longitudinal peripheral blood samples from 7 HIV-1/HTLV-1 coinfected individuals, and from 12 HIV-1 and 13 HTLV-1 mono-infected individuals. The proviral loads (PVL) were quantified using real-time PCR. Viral ISs and clonality were quantified by ligation-mediated PCR followed by high-throughput sequencing.
Results: The PVL of both HIV-1 and HTLV-1 in coinfected individuals was significantly higher than that of the respective virus in mono-infected individuals. The degree of oligoclonality of both HIV-1- and HTLV-1-infected cells in co-infected individuals was also greater than that in mono-infected subjects. The ISs of HIV-1 in cases of coinfection were more frequently located in intergenic regions and transcriptionally silent regions, compared with HIV-1 mono-infected individuals.
Conclusion: HIV-1/HTLV-1 coinfection makes an impact on the distribution of viral ISs and the clonality of virus-infected cells and thus may alter the risks of both HTLV-1- and HIV-1-associated disease.抄録：英語フィールド
Author：Hiroo Katsuya, Lucy B M Cook, Aileen G Rowan, Anat Melamed, Jocelyn Turpin, Jumpei Ito, Saiful Islam, Paola Miyazato, Benjy Jek Yang Tan, Misaki Matsuo, Toshikazu Miyakawa, Hirotomo Nakata, Shuzo Matsushita, Graham P Taylor, Charles R M Bangham, Shinya Kimura, Yorifumi SatouTitle：Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals Announcement information:J Infect Dis Vol: 225 Issue: 2 Page: 317-326Keyword：HIV-1; HTLV-1; High-throughput sequencing; Integration site analysis; coinfectionAn abstract：Background: Coinfection with HIV-1 and HTLV-1 diminishes the value of the CD4 + T-cell count in diagnosing AIDS, and increases the rate of HTLV-1-associated myelopathy. It remains elusive how HIV-1/HTLV-1 coinfection is related to such clinical characteristics. Here, we investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites (ISs) and the clonal expansion.
Methods: We extracted DNA from longitudinal peripheral blood samples from 7 HIV-1/HTLV-1 coinfected individuals, and from 12 HIV-1 and 13 HTLV-1 mono-infected individuals. The proviral loads (PVL) were quantified using real-time PCR. Viral ISs and clonality were quantified by ligation-mediated PCR followed by high-throughput sequencing.
Results: The PVL of both HIV-1 and HTLV-1 in coinfected individuals was significantly higher than that of the respective virus in mono-infected individuals. The degree of oligoclonality of both HIV-1- and HTLV-1-infected cells in co-infected individuals was also greater than that in mono-infected subjects. The ISs of HIV-1 in cases of coinfection were more frequently located in intergenic regions and transcriptionally silent regions, compared with HIV-1 mono-infected individuals.
Conclusion: HIV-1/HTLV-1 coinfection makes an impact on the distribution of viral ISs and the clonality of virus-infected cells and thus may alter the risks of both HTLV-1- and HIV-1-associated disease.