日本語フィールド
著者：Yoshioka, Mino; Fukuishi, Nobuyuki; Iriguchi, Sayuri; Ohsaki, Kanae; Yamanobe, Hiroyuki; Inukai, Asumi; Kurihara, Daisuke; Imajo, Naoki; Yasui, Yumiko; Matsui, Nobuaki; Tsujita, Tadayuki; Ishii, Akihiro; Seya, Tsukasa; Takahama, Makoto; Akagi, Masaaki題名：Lipoteichoic acid downregulates Fcε{lunate}RI expression on human mast cells through Toll-like receptor 2発表情報：Journal of Allergy and Clinical Immunology 巻: 120 号: 2 ページ: 452-461キーワード：概要：Background: Fcε{lunate}RI on the surface of mast cells (MCs) plays a central role in allergic responses. Recent evidence shows that exposure to microbial components corresponds with a significant reduction in the risk for allergic diseases. Although many reports suggest that this is due to changes in T-cell functions, how MC functions are altered by bacterial infection remains unknown. Objective: We sought to elucidate the effect of bacterial infection on MC function and expression of Fc receptors, such as Fcε{lunate}RI. Methods: Isolated human pulmonary MCs and a human MC line (LAD2) were stimulated with bacterial components, and the function and surface expression of Fc receptors were measured. Results: Lipoteichoic acid (LTA) and peptidoglycan, but not LPS, flagellin, or 3CpG-oligodeoxynucleotide, reduced the expression of Fcε{lunate}RI on LAD2 cells. An antibody to Toll-like receptor (TLR) 2 partially blocked the effect of LTA but not peptidoglycan. Both LTA and peptidoglycan reduced MC degranulation caused by an antigen-specific IgE. Furthermore, exposure of pulmonary MCs to LTA reduced both Fcε{lunate}RI expression and IgE-induced degranulation. None of the bacterial components affected the expression of other Fc receptors, such as Fcγ receptors or Fcα receptor I. Conclusions: Our results indicate that LTA reduces the surface expression of Fcε{lunate}RI through TLR2 and suggests that TLR2 ligands could be used as a novel therapy for controlling allergic disorders. Clinical implications: By knowing how bacterial components modulate MC function, we can expand our possibilities for therapeutic interventions of allergic diseases. © 2007 American Academy of Allergy, Asthma & Immunology.抄録：英語フィールド
Author：Yoshioka, Mino; Fukuishi, Nobuyuki; Iriguchi, Sayuri; Ohsaki, Kanae; Yamanobe, Hiroyuki; Inukai, Asumi; Kurihara, Daisuke; Imajo, Naoki; Yasui, Yumiko; Matsui, Nobuaki; Tsujita, Tadayuki; Ishii, Akihiro; Seya, Tsukasa; Takahama, Makoto; Akagi, MasaakiTitle：Lipoteichoic acid downregulates Fcε{lunate}RI expression on human mast cells through Toll-like receptor 2Announcement information:Journal of Allergy and Clinical Immunology Vol: 120 Issue: 2 Page: 452-461An abstract：Background: Fcε{lunate}RI on the surface of mast cells (MCs) plays a central role in allergic responses. Recent evidence shows that exposure to microbial components corresponds with a significant reduction in the risk for allergic diseases. Although many reports suggest that this is due to changes in T-cell functions, how MC functions are altered by bacterial infection remains unknown. Objective: We sought to elucidate the effect of bacterial infection on MC function and expression of Fc receptors, such as Fcε{lunate}RI. Methods: Isolated human pulmonary MCs and a human MC line (LAD2) were stimulated with bacterial components, and the function and surface expression of Fc receptors were measured. Results: Lipoteichoic acid (LTA) and peptidoglycan, but not LPS, flagellin, or 3CpG-oligodeoxynucleotide, reduced the expression of Fcε{lunate}RI on LAD2 cells. An antibody to Toll-like receptor (TLR) 2 partially blocked the effect of LTA but not peptidoglycan. Both LTA and peptidoglycan reduced MC degranulation caused by an antigen-specific IgE. Furthermore, exposure of pulmonary MCs to LTA reduced both Fcε{lunate}RI expression and IgE-induced degranulation. None of the bacterial components affected the expression of other Fc receptors, such as Fcγ receptors or Fcα receptor I. Conclusions: Our results indicate that LTA reduces the surface expression of Fcε{lunate}RI through TLR2 and suggests that TLR2 ligands could be used as a novel therapy for controlling allergic disorders. Clinical implications: By knowing how bacterial components modulate MC function, we can expand our possibilities for therapeutic interventions of allergic diseases. © 2007 American Academy of Allergy, Asthma & Immunology.