日本語フィールド
著者：Horitani, Masaki; Shisler, Krista; Broderick, William E.; Hutcheson, Rachel U.; Duschene, Kaitlin S.; Marts, Amy R.; Hoffman, Brian M.; Broderick, Joan B.題名：Metalloproteins: Radical SAM catalysis via an organometallic intermediate with an Fe-[5′-C]-deoxyadenosyl bond発表情報：Science 巻: 352 号: 6287 ページ: 822-825キーワード：概要：Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to cleave SAM to initiate diverse radical reactions. These reactions are thought to involve the 5′-deoxyadenosyl radical intermediate, which has not yet been detected. We used rapid freeze-quenching to trap a catalytically competent intermediate in the reaction catalyzed by the radical SAM enzyme pyruvate formate-lyase activating enzyme. Characterization of the intermediate by electron paramagnetic resonance and 13C, 57Fe electron nuclear double-resonance spectroscopies reveals that it contains an organometallic center in which the 5′ carbon of a SAM-derived deoxyadenosyl moiety forms a bond with the unique iron site of the [4Fe-4S] cluster. Discovery of this intermediate extends the list of enzymatic bioorganometallic centers to the radical SAM enzymes, the largest enzyme superfamily known, and reveals intriguing parallels to B12 radical enzymes.抄録：英語フィールド
Author：Horitani, Masaki; Shisler, Krista; Broderick, William E.; Hutcheson, Rachel U.; Duschene, Kaitlin S.; Marts, Amy R.; Hoffman, Brian M.; Broderick, Joan B.Title：Metalloproteins: Radical SAM catalysis via an organometallic intermediate with an Fe-[5′-C]-deoxyadenosyl bondAnnouncement information:Science Vol: 352 Issue: 6287 Page: 822-825An abstract：Radical S-adenosylmethionine (SAM) enzymes use a [4Fe-4S] cluster to cleave SAM to initiate diverse radical reactions. These reactions are thought to involve the 5′-deoxyadenosyl radical intermediate, which has not yet been detected. We used rapid freeze-quenching to trap a catalytically competent intermediate in the reaction catalyzed by the radical SAM enzyme pyruvate formate-lyase activating enzyme. Characterization of the intermediate by electron paramagnetic resonance and 13C, 57Fe electron nuclear double-resonance spectroscopies reveals that it contains an organometallic center in which the 5′ carbon of a SAM-derived deoxyadenosyl moiety forms a bond with the unique iron site of the [4Fe-4S] cluster. Discovery of this intermediate extends the list of enzymatic bioorganometallic centers to the radical SAM enzymes, the largest enzyme superfamily known, and reveals intriguing parallels to B12 radical enzymes.