日本語フィールド
著者：*Ritsuko Matsumura, Kazuto Yoshimi, Yuka Sawai, Nanami Yasumune, Kohhei Kajihara, Tatsuya Maejima, Tsuyoshi Koide, Koichi Node, Makoto Akashi題名：The role of cell-autonomous circadian oscillation of Cry transcription in circadian rhythm generation 発表情報：Cell Rep 巻: 39 号: 3 ページ: 110703キーワード：CP: Cell biology; circadian clock; circadian oscillator; circadian rhythm; clock gene; cryptochrome; period; protein degradation; protein half-life; transcription概要：The current model of the mammalian circadian clock describes cell-autonomous and negative feedback-driven circadian oscillation of Cry and Per transcription as the core circadian rhythm generator. However, the actual contribution of this oscillation to circadian rhythm generation remains undefined. Here we perform targeted disruption of cis elements indispensable for cell-autonomous Cry oscillation. Mice lacking overt cell-autonomous Cry oscillation show robust circadian rhythms in locomotor activity. In addition, tissue-autonomous circadian rhythms are robust in the absence of overt Cry oscillation. Unexpectedly, although the absence of overt Cry oscillation leads to severe attenuation of Per oscillation at the cell-autonomous level, circadian rhythms in Per2 accumulation remain robust. As a mechanism to explain this counterintuitive result, Per2 half-life shows cell-autonomous circadian rhythms independent of Cry and Per oscillation. The cell-autonomous circadian clock may therefore remain partially functional even in the absence of overt Cry and Per oscillation because of circadian oscillation in Per2 degradation. 抄録：英語フィールド
Author：*Ritsuko Matsumura, Kazuto Yoshimi, Yuka Sawai, Nanami Yasumune, Kohhei Kajihara, Tatsuya Maejima, Tsuyoshi Koide, Koichi Node, Makoto AkashiTitle：The role of cell-autonomous circadian oscillation of Cry transcription in circadian rhythm generation Announcement information:Cell Rep Vol: 39 Issue: 3 Page: 110703Keyword：CP: Cell biology; circadian clock; circadian oscillator; circadian rhythm; clock gene; cryptochrome; period; protein degradation; protein half-life; transcriptionAn abstract：The current model of the mammalian circadian clock describes cell-autonomous and negative feedback-driven circadian oscillation of Cry and Per transcription as the core circadian rhythm generator. However, the actual contribution of this oscillation to circadian rhythm generation remains undefined. Here we perform targeted disruption of cis elements indispensable for cell-autonomous Cry oscillation. Mice lacking overt cell-autonomous Cry oscillation show robust circadian rhythms in locomotor activity. In addition, tissue-autonomous circadian rhythms are robust in the absence of overt Cry oscillation. Unexpectedly, although the absence of overt Cry oscillation leads to severe attenuation of Per oscillation at the cell-autonomous level, circadian rhythms in Per2 accumulation remain robust. As a mechanism to explain this counterintuitive result, Per2 half-life shows cell-autonomous circadian rhythms independent of Cry and Per oscillation. The cell-autonomous circadian clock may therefore remain partially functional even in the absence of overt Cry and Per oscillation because of circadian oscillation in Per2 degradation. 