日本語フィールド
著者：Seto, Hirokazu; Kawakita, Hidetaka; Ohto, Keisuke; Harada, Hiroyuki; Inoue, Katsutoshi.題名： Membrane porosity control with dextran produced by immobilized dextransucrase.発表情報： Journal of Chemical Technology and Biotechnology 巻: 82 ページ: 248-252キーワード：概要：抄録：The porosity of Shirasu Porous Glass (SPG) membranes was controlled continuously with dextran produced by the enzymic reaction of dextransucrase (DSase). DSase was immobilized inside the pores of an SPG membrane at a d. of 1.5 U g-1, and a sucrose soln. was subsequently permeated through the DSase-immobilized SPG membrane to produce dextran from the active site of DSase. Varying the sucrose concn. from 5 to 25 mg L-1, resulted in a change in the rate of dextran produced, due to a decrease in the intermediate formation of the substrate-enzyme complex. Using the Kozeny-Carman equation, and based on the relationship between the pressure loss and pure water permeation rate, the membrane porosity could be varied between 38 and 66% by changing the amt. of dextran produced.英語フィールド
Author：Seto, Hirokazu; Kawakita, Hidetaka; Ohto, Keisuke; Harada, Hiroyuki; Inoue, Katsutoshi.Title： Membrane porosity control with dextran produced by immobilized dextransucrase.Announcement information: Journal of Chemical Technology and Biotechnology Vol: 82 Page: 248-252An abstract：The porosity of Shirasu Porous Glass (SPG) membranes was controlled continuously with dextran produced by the enzymic reaction of dextransucrase (DSase). DSase was immobilized inside the pores of an SPG membrane at a d. of 1.5 U g-1, and a sucrose soln. was subsequently permeated through the DSase-immobilized SPG membrane to produce dextran from the active site of DSase. Varying the sucrose concn. from 5 to 25 mg L-1, resulted in a change in the rate of dextran produced, due to a decrease in the intermediate formation of the substrate-enzyme complex. Using the Kozeny-Carman equation, and based on the relationship between the pressure loss and pure water permeation rate, the membrane porosity could be varied between 38 and 66% by changing the amt. of dextran produced.