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Construction and characterization of a BAC library of soybean.

発表形態:
原著論文
主要業績:
その他
単著・共著:
共著
発表年月:
2005年
DOI:
会議属性:
指定なし
査読:
有り
リンク情報:

日本語フィールド

著者:
Zhengjun Xia, Hiroko Sato, Satoshi Watanabe, Shiji Kawasaki and Kyuya Harada
題名:
Construction and characterization of a BAC library of soybean.
発表情報:
Euphytica 巻: 141 ページ: 129-137
キーワード:
概要:
抄録:
We have constructed a soybean (Glycine max (L.) Merrill) bacterial artificial chromosome (BAC) library from green leaf protoplasts of the cultivar, Misuzudaizu. The library contains 53,760 clones with an average insert size of 116 kb. About 2.9% chloroplast DNA origin was revealed by PCR and colony hybridization. Apart from 2.8% clones having no insert, this library represents 5.2 genome equivalents. With this genome coverage, the probability of having any DNA sequence represented in the library is higher than 99.5%. Three-dimensional pools of the BAC library in combination with the use of a high efficiency genome scanning (HEGS) electrophoresis facilitate rapid and efficient PCR-based screening. An average of five positive clones were identified after screening the BAC library with SSR and STS markers. BAC-end walking was performed for three SSR associated BACs. This library will provide a good resource for positional cloning of agronomically and biologically important QTL genes that Misuzudaizu possesses.

英語フィールド

Author:
Zhengjun Xia, Hiroko Sato, Satoshi Watanabe, Shiji Kawasaki and Kyuya Harada
Title:
Construction and characterization of a BAC library of soybean.
Announcement information:
Euphytica Vol: 141 Page: 129-137
An abstract:
We have constructed a soybean (Glycine max (L.) Merrill) bacterial artificial chromosome (BAC) library from green leaf protoplasts of the cultivar, Misuzudaizu. The library contains 53,760 clones with an average insert size of 116 kb. About 2.9% chloroplast DNA origin was revealed by PCR and colony hybridization. Apart from 2.8% clones having no insert, this library represents 5.2 genome equivalents. With this genome coverage, the probability of having any DNA sequence represented in the library is higher than 99.5%. Three-dimensional pools of the BAC library in combination with the use of a high efficiency genome scanning (HEGS) electrophoresis facilitate rapid and efficient PCR-based screening. An average of five positive clones were identified after screening the BAC library with SSR and STS markers. BAC-end walking was performed for three SSR associated BACs. This library will provide a good resource for positional cloning of agronomically and biologically important QTL genes that Misuzudaizu possesses.


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