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An Integrated High-density Linkage Map of Soybean with RFLP, SSR, STS, and AFLP Markers Using A Single F2 Population.

発表形態:
原著論文
主要業績:
その他
単著・共著:
共著
発表年月:
2007年
DOI:
会議属性:
指定なし
査読:
有り
リンク情報:

日本語フィールド

著者:
Zhengjun Xia, Yasutaka Tsubokura, Masako Hoshi, Masayoshi Hanawa, Chizuru Yano, Kayo Okamura, Talaat A. Ahmed, Toyoaki Anai, Satoshi Watanabe, Masaki Hayashi, Takashi Kawai, Khwaja G. Hossain, Hirokazu Masaki, Kazumi Asai, Naoki Yamanaka, Nakao Kubo, Koh-ichi Kadowaki, Yoshiaki Nagamura, Masahiro Yano, Takuji Sasaki, and Kyuya Harada
題名:
An Integrated High-density Linkage Map of Soybean with RFLP, SSR, STS, and AFLP Markers Using A Single F2 Population.
発表情報:
DNA Research 巻: 14 ページ: 257-269
キーワード:
概要:
抄録:
Soybean [Glycine max (L.) Merrill] is the most important leguminous crop in the world due to its high contents of high-quality protein and oil for human and animal consumption as well as for industrial uses. An accurate and saturated genetic linkage map of soybean is an essential tool for studies on modern soybean genomics. In order to update the linkage map of a F2 population derived from a cross between Misuzudaizu and Moshidou Gong 503 and to make it more informative and useful to the soybean genome research community, a total of 318 AFLP, 121 SSR, 108 RFLP, and 126 STS markers were newly developed and integrated into the framework of the previously described linkage map. The updated genetic map is composed of 509 RFLP, 318 SSR, 318 AFLP, 97 AFLP-derived STS, 29 BAC-end or EST-derived STS, 1 RAPD, and five morphological markers, covering a map distance of 3080 cM (Kosambi function) in 20 linkage groups (LGs). To our knowledge, this is presently the densest linkage map developed from a single F2 population in soybean. The average intermarker distance was reduced to 2.41 from 5.78 cM in the earlier version of the linkage map. Most SSR and RFLP markers were relatively evenly distributed among different LGs in contrast to the moderately clustered AFLP markers. The number of gaps of more than 25 cM was reduced to 6 from 19 in the earlier version of the linkage map. The coverage of the linkage map was extended since 17 markers were mapped beyond the distal ends of the previous linkage map. In particular, 17 markers were tagged in a 5.7 cM interval between CE47M5a and Satt100 on LG C2, where several important QTLs were clustered. This newly updated soybean linkage map will enable to streamline positional cloning of agronomically important trait locus genes, and promote the development of physical maps, genome sequencing, and other genomic research activities.

英語フィールド

Author:
Zhengjun Xia, Yasutaka Tsubokura, Masako Hoshi, Masayoshi Hanawa, Chizuru Yano, Kayo Okamura, Talaat A. Ahmed, Toyoaki Anai, Satoshi Watanabe, Masaki Hayashi, Takashi Kawai, Khwaja G. Hossain, Hirokazu Masaki, Kazumi Asai, Naoki Yamanaka, Nakao Kubo, Koh-ichi Kadowaki, Yoshiaki Nagamura, Masahiro Yano, Takuji Sasaki, and Kyuya Harada
Title:
An Integrated High-density Linkage Map of Soybean with RFLP, SSR, STS, and AFLP Markers Using A Single F2 Population.
Announcement information:
DNA Research Vol: 14 Page: 257-269
An abstract:
Soybean [Glycine max (L.) Merrill] is the most important leguminous crop in the world due to its high contents of high-quality protein and oil for human and animal consumption as well as for industrial uses. An accurate and saturated genetic linkage map of soybean is an essential tool for studies on modern soybean genomics. In order to update the linkage map of a F2 population derived from a cross between Misuzudaizu and Moshidou Gong 503 and to make it more informative and useful to the soybean genome research community, a total of 318 AFLP, 121 SSR, 108 RFLP, and 126 STS markers were newly developed and integrated into the framework of the previously described linkage map. The updated genetic map is composed of 509 RFLP, 318 SSR, 318 AFLP, 97 AFLP-derived STS, 29 BAC-end or EST-derived STS, 1 RAPD, and five morphological markers, covering a map distance of 3080 cM (Kosambi function) in 20 linkage groups (LGs). To our knowledge, this is presently the densest linkage map developed from a single F2 population in soybean. The average intermarker distance was reduced to 2.41 from 5.78 cM in the earlier version of the linkage map. Most SSR and RFLP markers were relatively evenly distributed among different LGs in contrast to the moderately clustered AFLP markers. The number of gaps of more than 25 cM was reduced to 6 from 19 in the earlier version of the linkage map. The coverage of the linkage map was extended since 17 markers were mapped beyond the distal ends of the previous linkage map. In particular, 17 markers were tagged in a 5.7 cM interval between CE47M5a and Satt100 on LG C2, where several important QTLs were clustered. This newly updated soybean linkage map will enable to streamline positional cloning of agronomically important trait locus genes, and promote the development of physical maps, genome sequencing, and other genomic research activities.


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