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Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library.

発表形態:
原著論文
主要業績:
その他
単著・共著:
共著
発表年月:
2014年
DOI:
会議属性:
指定なし
査読:
有り
リンク情報:

日本語フィールド

著者:
Xia Z, Wu H, Watanabe S, Harada K.
題名:
Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library.
発表情報:
Analytical Biochemistry 巻: 444 ページ: 38-40
キーワード:
概要:
抄録:
Abstract Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.

英語フィールド

Author:
Xia Z, Wu H, Watanabe S, Harada K.
Title:
Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library.
Announcement information:
Analytical Biochemistry Vol: 444 Page: 38-40
An abstract:
Abstract Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.


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