日本語フィールド
著者：Kazutoshi Nishijima, Yosuke Yamada, Hiroaki Tanaka, Shuji Kitajima, Shinji Yamaguchi, Masatoshi Morimoto, Keitaro Tanaka, and Yuichiro Nishida.題名：Assessment of energy expenditure in rabbit with doubly-labeled water method発表情報：Selected short presentation (Proceedings page 40). 4th International Rabbit Biotechnology Meeting, Budapest, 30th June – 1st July 2011.キーワード：概要：抄録：Rabbit can become a relevant animal model to research human metabolic syndrome and obesity, since rabbit has several characteristics similar to human in metabolism. Analysis of the energy metabolism is essential to clarify mechanisms of obesity. Doubly-labeled water (DLW; 2H2O and H218O) method has been widely accepted as a gold standard method to assess energy expenditure and body composition in wild animals and free-living or hospitalized humans without any restraint. However, to our knowledge, there is no previous study which applied the DLW method into rabbit studies. Thus, we investigated the application of DLW method to develop an optimum assessment of energy expenditure in rabbits. DLW was injected (0.92g/kg body weight, i.v.) into Japanese White rabbits (male, 26 weeks old). Blood samples were collected before and 3-4 hours and 4, 7, 8, 10, 14, and 15 days after the injection. The enrichment of 2H and 18O in plasma samples were analyzed with an isotope ratio mass spectrometry. Carbon dioxide production rate was calculated by measuring the difference between the elimination rates of 2H and 18O, and converted into the energy expenditure. Body composition was also estimated by isotope dilution space of 2H and 18O. The contribution ratio (R2) of the linear regression, between natural logarithm transformed enrichments and days after DLW injection, was 0.998 � 0.002 (mean � SD) for 2H and 0.999 � 0.001 for 18O. The energy expenditure was 340.1 � 9.3 kcal/day. Total body water and body fat percentage were estimated to be 2.3 � 0.1kg and 16.0 � 1.4%. The present results showed that usage of the current dose (0.92g/kg) and 15 days of experimental period can be used to precisely access the energy metabolism in rabbits.英語フィールド
Author：Kazutoshi Nishijima, Yosuke Yamada, Hiroaki Tanaka, Shuji Kitajima, Shinji Yamaguchi, Masatoshi Morimoto, Keitaro Tanaka, and Yuichiro Nishida.Title：Assessment of energy expenditure in rabbit with doubly-labeled water methodAnnouncement information:Selected short presentation (Proceedings page 40). 4th International Rabbit Biotechnology Meeting, Budapest, 30th June – 1st July 2011.An abstract：Rabbit can become a relevant animal model to research human metabolic syndrome and obesity, since rabbit has several characteristics similar to human in metabolism. Analysis of the energy metabolism is essential to clarify mechanisms of obesity. Doubly-labeled water (DLW; 2H2O and H218O) method has been widely accepted as a gold standard method to assess energy expenditure and body composition in wild animals and free-living or hospitalized humans without any restraint. However, to our knowledge, there is no previous study which applied the DLW method into rabbit studies. Thus, we investigated the application of DLW method to develop an optimum assessment of energy expenditure in rabbits. DLW was injected (0.92g/kg body weight, i.v.) into Japanese White rabbits (male, 26 weeks old). Blood samples were collected before and 3-4 hours and 4, 7, 8, 10, 14, and 15 days after the injection. The enrichment of 2H and 18O in plasma samples were analyzed with an isotope ratio mass spectrometry. Carbon dioxide production rate was calculated by measuring the difference between the elimination rates of 2H and 18O, and converted into the energy expenditure. Body composition was also estimated by isotope dilution space of 2H and 18O. The contribution ratio (R2) of the linear regression, between natural logarithm transformed enrichments and days after DLW injection, was 0.998 � 0.002 (mean � SD) for 2H and 0.999 � 0.001 for 18O. The energy expenditure was 340.1 � 9.3 kcal/day. Total body water and body fat percentage were estimated to be 2.3 � 0.1kg and 16.0 � 1.4%. The present results showed that usage of the current dose (0.92g/kg) and 15 days of experimental period can be used to precisely access the energy metabolism in rabbits.