日本語フィールド
著者：Kartika A. Madurani, Suprapto Suprapto, Muhammad Yudha Syahputra, Ika Puspita, Abdul Hadi Furqoni, Kistya Puspasari, Hafildatu Rosyidah, Agus Muhamad Hatta, Juniastuti Juniastuti, Maria Inge Lusida, Masato Tominaga, Fredy Kurniawan題名：Fluorescence Spectrophotometry for COVID-19 Determination in Clinical Swab Samples発表情報：Arabian Journal of Chemistry 巻: 15 ページ: article No. 104020キーワード：概要：抄録：Considering the limitations of the assays currently available for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its emerging variants, a simple and rapid method using fluorescence spectrophotometry was developed to detect coronavirus disease 2019 (COVID-19). Forty clinical swab samples were collected from the nasopharyngeal and oropharyngeal cavities of COVID-19-positive and -negative. Each sample was divided into two parts. The first part of the samples was analyzed using reverse transcription-polymerase chain reaction (RT-qPCR) as the control method to identify COVID-19-positive and -negative samples. The second part of the samples was analyzed using fluorescence spectrophotometry. Fluorescence measurements were performed at excitation and emission wavelengths ranging from 200 to 800 nm. Twenty COVID-19-positive samples and twenty COVID-19 negative samples were detected based on RT-qPCR results. The fluorescence spectrum data indicated that the COVID-19-positive and negative samples had significantly different characteristics. All positive samples could be distinguished from negative samples by fluorescence spectrophotometry. Principal component analysis showed that COVID-19-positive samples were clustered separately from COVID-19-negative samples. The specificity and accuracy of this experiment reached 100%. Limit of detection (LOD) obtained 42.20 copies/ml (Ct value of 33.65 cycles) for E gene and 63.60 copies/ml (Ct value of 31.36 cycles) for ORF1ab gene. This identification process only required 4 min. Thus, this technique offers an efficient and accurate method to identify an individual with active SARS-CoV-2 infection and can be easily adapted for the early investigation of COVID-19, in general.英語フィールド
Author：Kartika A. Madurani, Suprapto Suprapto, Muhammad Yudha Syahputra, Ika Puspita, Abdul Hadi Furqoni, Kistya Puspasari, Hafildatu Rosyidah, Agus Muhamad Hatta, Juniastuti Juniastuti, Maria Inge Lusida, Masato Tominaga, Fredy KurniawanTitle：Fluorescence Spectrophotometry for COVID-19 Determination in Clinical Swab SamplesAnnouncement information:Arabian Journal of Chemistry Vol: 15 Page: article No. 104020An abstract：Considering the limitations of the assays currently available for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its emerging variants, a simple and rapid method using fluorescence spectrophotometry was developed to detect coronavirus disease 2019 (COVID-19). Forty clinical swab samples were collected from the nasopharyngeal and oropharyngeal cavities of COVID-19-positive and -negative. Each sample was divided into two parts. The first part of the samples was analyzed using reverse transcription-polymerase chain reaction (RT-qPCR) as the control method to identify COVID-19-positive and -negative samples. The second part of the samples was analyzed using fluorescence spectrophotometry. Fluorescence measurements were performed at excitation and emission wavelengths ranging from 200 to 800 nm. Twenty COVID-19-positive samples and twenty COVID-19 negative samples were detected based on RT-qPCR results. The fluorescence spectrum data indicated that the COVID-19-positive and negative samples had significantly different characteristics. All positive samples could be distinguished from negative samples by fluorescence spectrophotometry. Principal component analysis showed that COVID-19-positive samples were clustered separately from COVID-19-negative samples. The specificity and accuracy of this experiment reached 100%. Limit of detection (LOD) obtained 42.20 copies/ml (Ct value of 33.65 cycles) for E gene and 63.60 copies/ml (Ct value of 31.36 cycles) for ORF1ab gene. This identification process only required 4 min. Thus, this technique offers an efficient and accurate method to identify an individual with active SARS-CoV-2 infection and can be easily adapted for the early investigation of COVID-19, in general.