日本語フィールド
著者:Md. Mizanur Rahim KHAN, Masaki IWAYOSHI, Yuki OGURA-TSUJITA and Shiro ISSHIKI(Corresponding author)題名:Genetic characterization of the fertility restorer (Rf) genes and their linked DNA marker of the male sterile eggplant systems having the cytoplasm of the wild species発表情報:Environ. Control Biol. 巻: 56 号: 4 ページ: 173-176キーワード:概要:The fertility restorer (Rf) genes and their linked DNA marker (the SCAB101900 marker) of the male sterile eggplant systems having the cytoplasm of the three wild species, Solanum aethiopicum L. Aculeatum Group, S. anguivi Lam. and S. grandifolium C. V. Morton were genetically characterized. The backcross 8th generation (BC8), BC7 and BC8 with S. anguivi, S. grandifolium and S. aethiopicum cytoplasm parents, respectively, segregated for male fertile and sterile in a 3 : 1 ratio, which corresponds to two independent dominant Rf gene model. Presence/absence of the SCAB101900 marker for male fertility and sterility was analyzed in the three backcross progenies. The marker appeared in all the male fertile individuals, except one BC7 plant with S. grandifolium cytoplasm parent. These indicate that this SCAB101900 marker is very closely linked to both of the Rf loci. There would be no quantitative effect of the Rf genes on pollen fertility. If there is at least one Rf dominant allele at any of the 2 loci, pollen seems to be fertile. As a result of detecting the SCAB101900 marker on 7 wild species, S. gilo was revealed to have these Rf and the same male sterile cytoplasm.抄録:The fertility restorer (Rf) genes and their linked DNA marker (the SCAB101900 marker) of the male sterile eggplant systems having the cytoplasm of the three wild species, Solanum aethiopicum L. Aculeatum Group, S. anguivi Lam. and S. grandifolium C. V. Morton were genetically characterized. The backcross 8th generation (BC8), BC7 and BC8 with S. anguivi, S. grandifolium and S. aethiopicum cytoplasm parents, respectively, segregated for male fertile and sterile in a 3 : 1 ratio, which corresponds to two independent dominant Rf gene model. Presence/absence of the SCAB101900 marker for male fertility and sterility was analyzed in the three backcross progenies. The marker appeared in all the male fertile individuals, except one BC7 plant with S. grandifolium cytoplasm parent. These indicate that this SCAB101900 marker is very closely linked to both of the Rf loci. There would be no quantitative effect of the Rf genes on pollen fertility. If there is at least one Rf dominant allele at any of the 2 loci, pollen seems to be fertile. As a result of detecting the SCAB101900 marker on 7 wild species, S. gilo was revealed to have these Rf and the same male sterile cytoplasm.英語フィールド
Author:Md. Mizanur Rahim KHAN, Masaki IWAYOSHI, Yuki OGURA-TSUJITA and Shiro ISSHIKI(Corresponding author)Title:Genetic characterization of the fertility restorer (Rf) genes and their linked DNA marker of the male sterile eggplant systems having the cytoplasm of the wild speciesAnnouncement information:Environ. Control Biol. Vol: 56 Issue: 4 Page: 173-176An abstract:The fertility restorer (Rf) genes and their linked DNA marker (the SCAB101900 marker) of the male sterile eggplant systems having the cytoplasm of the three wild species, Solanum aethiopicum L. Aculeatum Group, S. anguivi Lam. and S. grandifolium C. V. Morton were genetically characterized. The backcross 8th generation (BC8), BC7 and BC8 with S. anguivi, S. grandifolium and S. aethiopicum cytoplasm parents, respectively, segregated for male fertile and sterile in a 3 : 1 ratio, which corresponds to two independent dominant Rf gene model. Presence/absence of the SCAB101900 marker for male fertility and sterility was analyzed in the three backcross progenies. The marker appeared in all the male fertile individuals, except one BC7 plant with S. grandifolium cytoplasm parent. These indicate that this SCAB101900 marker is very closely linked to both of the Rf loci. There would be no quantitative effect of the Rf genes on pollen fertility. If there is at least one Rf dominant allele at any of the 2 loci, pollen seems to be fertile. As a result of detecting the SCAB101900 marker on 7 wild species, S. gilo was revealed to have these Rf and the same male sterile cytoplasm.An abstract:The fertility restorer (Rf) genes and their linked DNA marker (the SCAB101900 marker) of the male sterile eggplant systems having the cytoplasm of the three wild species, Solanum aethiopicum L. Aculeatum Group, S. anguivi Lam. and S. grandifolium C. V. Morton were genetically characterized. The backcross 8th generation (BC8), BC7 and BC8 with S. anguivi, S. grandifolium and S. aethiopicum cytoplasm parents, respectively, segregated for male fertile and sterile in a 3 : 1 ratio, which corresponds to two independent dominant Rf gene model. Presence/absence of the SCAB101900 marker for male fertility and sterility was analyzed in the three backcross progenies. The marker appeared in all the male fertile individuals, except one BC7 plant with S. grandifolium cytoplasm parent. These indicate that this SCAB101900 marker is very closely linked to both of the Rf loci. There would be no quantitative effect of the Rf genes on pollen fertility. If there is at least one Rf dominant allele at any of the 2 loci, pollen seems to be fertile. As a result of detecting the SCAB101900 marker on 7 wild species, S. gilo was revealed to have these Rf and the same male sterile cytoplasm.