日本語フィールド
著者：Khan, M.M.R. and S. Isshiki (Corresponding Author)題名：Development of the male sterile line of eggplant using the cytoplasm of Solanum aethiopicum Aculeatum Group発表情報：Journal of the Japanese Society for Horticultural Science 巻: 79 号: 4 ページ: 348-353キーワード：cytoplasmic male-sterility, eggplant, pollen non-formation, Solanum aethiopicum, Solanum melongena概要：抄録：ナス(Solanum melongena L.)の新規の雄性不稔系統を育成することを目的に，F1台木品種‘アシスト’[(Solanum aethiopicum Aculeatum Group) × ナス ‘DMP’]を細胞質親およびナス‘Uttara’を核親とする連続戻し交雑による細胞質置換を進め，戻し交雑第四代（BC4）まで作出した．花粉形成の有無を調査した結果，F1‘アシスト’は供試個体すべてが花粉を形成したのに対し，BC1では花粉を全く形成しない雄性不稔個体と雄性可稔個体に分離した．雄性可稔個体から得られたBC2，BC3およびBC4では雄性不稔個体と雄性可稔個体に分離し，その分離比は３：１に適合した．雄性不稔個体から得られたBC3およびBC4では分離が見られず，すべて雄性不稔個体であった．雄性可稔のBC2の自殖実生では雄性不稔個体と雄性可稔個体に分離し，その分離比は１５：１に適合した．これらのことから，S. aethiopicum Aculeatum Groupの細胞質がナスに花粉を形成しないタイプの雄性不稔性を誘起することが推定された．さらに，本雄性不稔性に対して優性の稔性回復遺伝子（Rf）が２つ存在することが推定された．種子稔性を調査した結果，BC4において結果率，１果あたりの種子数および種子発芽率のいずれもが高い値を示したことから，S. aethiopicum Aculeatum Groupの細胞質がナスの種子稔性に悪影響を及ぼさないことが示唆された．ナスの細胞質をS. aethiopicum Aculeatum Groupのもので置換することによって新規の雄性不稔系統を育成できた．英語フィールド
Author：Khan, M.M.R. and S. Isshiki (Corresponding Author)Title：Development of the male sterile line of eggplant using the cytoplasm of Solanum aethiopicum Aculeatum GroupAnnouncement information:Journal of the Japanese Society for Horticultural Science Vol: 79 Issue: 4 Page: 348-353Keyword：cytoplasmic male-sterility, eggplant, pollen non-formation, Solanum aethiopicum, Solanum melongenaAn abstract：In order to develop a new male-sterile line of eggplant (Solanum melongena L.), the F1 rootstock ‘Assist’ (Solanum aethiopicum Aculeatum Group × S. melongena ‘DMP’) was continuously backcrossed to S. melongena ‘Uttara’ using ‘Uttara’ as a recurrent pollen parent up to BC4 generation. All the examined F1 ‘Assist’ produced pollen grains while the BC1 plants segregated into male fertile and sterile plants. The male-sterile plant did not produce any pollen grains. The BC2, BC3 and BC4 progenies obtained from the male fertile backcross progenies were segregated into the male fertile and sterile plants and the each segregation ratio fitted to 3 : 1. The BC3 and BC4 progenies obtained from the male-sterile backcross progenies were fixed to the male-sterility without segregation. The selfed progenies of the male fertile BC2 progeny were segregated into the male fertile and sterile plants and the segregation ratio fitted to 15 : 1. Therefore, the pollen non-formation character is assumed to be a form of cytoplasmic male-sterility (CMS), induced by incompatibility between the cytoplasm of S. aethiopicum Aculeatum Group and the nucleus of S. melongena. Further, these results of the segregations indicated that two independent dominant fertility restorer (Rf) genes control the fertility restoration of this CMS. High values of fruit set, number of seeds per fruit and seed germination rate found in the BC4 progenies indicate that the cytoplasm of S. aethiopicum Aculeatum Group has no notable negative effect on seed fertility of S. melongena. A new male-sterile line of eggplant could be developed by utilizing the cytoplasm of S. aethiopicum Aculeatum Group.