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Drug Response Analysis for Scaffold-Free Cardiac Constructs Fabricated Using bio-3D Printer

発表形態:
原著論文
主要業績:
主要業績
単著・共著:
共著
発表年月:
2020年06月
DOI:
10.1038/s41598-020-65681-y
会議属性:
指定なし
査読:
有り
リンク情報:

日本語フィールド

著者:
*Arai K, Murata D, Takao S, Nakamura A, Itoh M, Kitsuka T, Nakayama K
題名:
Drug Response Analysis for Scaffold-Free Cardiac Constructs Fabricated Using bio-3D Printer
発表情報:
Sci Rep 巻: 10 号: 1 ページ: 8972
キーワード:
概要:
Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we fabricated scaffold-free three-dimensional (3D) tubular cardiac constructs using a bio-3D printer, which can load cardiac spheroids onto a needle array. In this study, we developed a method to measure the contractile force and to evaluate the drug response in cardiac constructs. Specifically, we measured the movement of the needle tip upon contraction of the cardiac constructs on the needle array. The contractile force and beating rate of the cardiac constructs were evaluated by analysing changes in the movement of the needle tip. To evaluate the drug response, contractile properties were measured following treatment with isoproterenol, propranolol, or blebbistatin, in which the movement of the needle tip was increased following isoproterenol treatment, but was decreased following propranolol or blebbistain, treatments. To evaluate cardiotoxicity, contraction and cell viability of the cardiac constructs were measured following doxorubicin treatment. Cell viability was found to decrease with decreasing movement of the needle tip following doxorubicin treatment. Collectively, our results show that this method can aid in evaluating the contractile force of cardiac constructs.
抄録:

英語フィールド

Author:
*Arai K, Murata D, Takao S, Nakamura A, Itoh M, Kitsuka T, Nakayama K
Title:
Drug Response Analysis for Scaffold-Free Cardiac Constructs Fabricated Using bio-3D Printer
Announcement information:
Sci Rep Vol: 10 Issue: 1 Page: 8972
An abstract:
Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we fabricated scaffold-free three-dimensional (3D) tubular cardiac constructs using a bio-3D printer, which can load cardiac spheroids onto a needle array. In this study, we developed a method to measure the contractile force and to evaluate the drug response in cardiac constructs. Specifically, we measured the movement of the needle tip upon contraction of the cardiac constructs on the needle array. The contractile force and beating rate of the cardiac constructs were evaluated by analysing changes in the movement of the needle tip. To evaluate the drug response, contractile properties were measured following treatment with isoproterenol, propranolol, or blebbistatin, in which the movement of the needle tip was increased following isoproterenol treatment, but was decreased following propranolol or blebbistain, treatments. To evaluate cardiotoxicity, contraction and cell viability of the cardiac constructs were measured following doxorubicin treatment. Cell viability was found to decrease with decreasing movement of the needle tip following doxorubicin treatment. Collectively, our results show that this method can aid in evaluating the contractile force of cardiac constructs.


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